Comparison of calculated and experimental power in maximal lactatesteady state during cycling
 Thomas Hauser^{1}Email author,
 Jennifer Adam^{1, 2} and
 Henry Schulz^{1}
DOI: 10.1186/174246821125
© Hauser et al.; licensee BioMed Central Ltd. 2014
Received: 5 February 2014
Accepted: 16 May 2014
Published: 27 May 2014
Abstract
Background
The purpose of this study was the comparison of the calculated (MLSS_{C}) and experimental power (MLSS_{E}) in maximal lactate steadystate (MLSS) during cycling.
Methods
13 male subjects (24.2 ± 4.76 years, 72.9 ± 6.9 kg, 178.5 ± 5.9 cm, : 60.4 ± 8.6 ml min^{−1} kg^{−1}, : 0.9 ± 0.19 mmol l^{1} s^{1}) performed a ramptest for determining the and a 15 s sprinttest for measuring the maximal glycolytic rate ( ). All tests were performed on a LodeCycleErgometer. and were used to calculate MLSS_{C}. For the determination of MLSS_{E} several 30 min constant load tests were performed. MLSS_{E} was defined as the highest workload that can be maintained without an increase of bloodlactateconcentration (BLC) of more than 0.05 mmol l^{−1} min^{−1} during the last 20 min. Power in following constantload test was set higher or lower depending on BLC.
Results
MLSS_{E} and MLSS_{C} were measured respectively at 217 ± 51 W and 229 ± 47 W, while mean difference was −12 ± 20 W. Orthogonal regression was calculated with r = 0.92 (p < 0.001).
Conclusions
The difference of 12 W can be explained by the biological variability of and . The knowledge of both parameters, as well as their individual influence on MLSS, could be important for establishing training recommendations, which could lead to either an improvement in or by performing high intensity or low intensity exercise training, respectively. Furthermore the validity of test should be focused in further studies.
Keywords
Maximal lactatesteadystate Calculation Lactateproduction rate Elimination of lactateIntroduction
Over the last 35 years, incremental graded exercise tests have been established for detecting endurance performance on the basis of a lactateperformance curve and the application of several different lactatethreshold concepts [1]. Most of these lactate concepts have the aim to approximate the power output achieved at maximal lactatesteadystate (PMLSS), which is one criterion of endurance performance [1, 2]. PMLSS is defined as the highest workload where lactateformation and lactateelimination in the muscle cell are maintained at a steadystate [2–4]. However, Hauser et al. [5] compared the power at "onset of blood lactate accumulation" (OBLA) [6, 7], the "individual anaerobic threshold" (IAT) [8] and the " + 1.5 mmol·l^{−1} lactate model" [9] with power in MLSS, measured during 30minutes constant load tests. They found high significant correlations between OBLA and MLSS: r = 0.89 (mean difference −7.4 W); IAT and MLSS: r = 0.83 (mean difference 12.4 W), +1.5 mmol·l^{−1} lactate model and MLSS: r = 0.88 (mean difference −37.4 W). However, based on BlandandAltman, the comparison of power of all thresholdconcepts with power in MLSS showed large individual differences, which deceive the high regression coefficients and small mean differences between these methods.
Furthermore, it is problematical that lactatethreshold concepts are based solely on the bloodlactateconcentration (BLC), which is mainly influenced by lactate formation, −transport, −diffusion and elimination. Therefore, BLC may not represent the true metabolic processes occurring within the muscle cell. Mader [10, 11] and Bleicher et al. [12] have previously suggested that the same lactateperformancecurve may result from different combinations of maximal oxygen uptake ( ) and maximal lactate production rate ( ). Furthermore, the shift of a lactateperformance curve could also be achieved by changing or separately.
Indeed, Bleicher et al. [12] verified, that two different athletes, (soccer and track), had exactly the same velocity for onset of blood lactate accumulation (OBLA) of 4.4 m s^{−1}, yet the individual parameters of and were higher for the soccer player when compared to the track athlete ( : 70 vs. 63 ml min^{−1} kg^{−1}; 0.93 vs. 0.65 mmol l^{−1} s^{−1}, respectively). That confirms, therefore that identical MLSS could be originate by completely different combinations of  and values. Using either , or BLC alone, it is not possible to explain differences of PMLSS between two athletes or the effects of training on the MLSS. As such, it would be beneficial to understand, how MLSS is controlled by glycolysis and oxidative phosphorylation within the muscle cell.
The present study, therefore, hypothesised firstly that it would be possible to calculate the PMLSS using the method by Mader and Heck [3] and secondly that knowledge of and (and their interaction) would help to better understand the mechanisms of the MLSS. These outcomes could provide a benefit compared to lactatethreshold concepts and to timeextensive 30 min constantload tests.
Methods
Study sample
13 male subjects (age: 24.2 ± 4.76 yr, weight: 72.9 ± 6.9 kg, height: 178.5 ± 5.9 cm, : 60.4 ± 8.6 ml·min^{−1}·kg^{−1}, : 0.9 ± 0.19 mmol l^{1} s^{1}) with different endurance levels participated in this study (training volume: n = 4 between 10 and 14 hours/week, n = 7 from 2 to 8 hours/week, n = 2 no sport). All subjects were informed about the aims of the study and subsequently provided written consent in accordance with the declaration of Helsinki [13].
Procedure
All tests were performed on a Lode Excalibur Sport Ergometer (Lode, Groningen, NL). At the beginning of this investigation subjects performed, in a random order, a test for detecting the maximal glycolytic rate and a test for detecting the maximal aerobic performance. Using the method introduced by Mader and Heck [3], PMLSS_{C} was calculated on the basis of the individual , and body weight. PMLSS_{C} was used for the first constantloadtest and several 30 min constant loadtests were undertaken to detect PMLSS_{E}. Each test was performed on different days.
Equation 1: Calculation of maximal glycolytic rate.
Abbreviations are as follows: La_{maxPost} = Maximal Post Exercise Bloodlactate, La_{Pre} = Bloodlactate before test, t_{test} = test duration = 15 sec, t_{alac} = alactic time interval
The alactic time interval (t_{alac}) was defined as the time from the beginning of the sprint (0 sec) to when the maximum power decreases by 3.5%.
Subjects performed a ramptest for measuring breathbybreath (Oxycon Pro, Jäger, Höchberg, Germany) which included a warm up of 10 minutes at a constant load corresponding to 1.5 times of the participant’s bodyweight, followed by a period of 2 min at a constant load of 50 W. The workload at the beginning of the test was set to 50 W for 2 min and was increased by 25 W every 30 s. The test was finished when subjects reached physically exhaustion, complaints of shortness of breath, dizziness or other physical complaints that unabled them proceeding the test [15]. was calculated by the mean of all values measured within the last 30s of the test.
Calculation of PMLSS_{C}
Step 1: Biochemical elementary background
Equation 2: Elementary equation of MichaelsMentenkinetics, where activation of an enzymesubstratecomplex ( ) depends on maximal performance ( ), 50%activityconstant (K_{M}) and substrate (S).
It is mostly agreed that under nomoxic conditions the main regulating substrate (S) for the activation of and is the level of free ADP concentration [3, 11, 16, 17]. With an increase of the workload and therefore a higher demand of ATP, ADPconcentration rises exponentially within the muscle towards and [11].
Step 2: Activation of oxidative phosphorylation ( )
Equation 3: Transformed equation of MichaelsMentenkinetics to calculate the activation of oxidative phosphorylation ( ) – depending on maximal oxygen uptake ( ), 50%activityconstant (Ks1) and substrate (ADP).
Step 3: Activation of glycolysis ( )
Step 4: Calculation of Lactateeliminationrate depending on
Equation 5: Calculation of maximal lactate elimination rate ( ) – depending on lactate equivalent, lactate distribution volume and activity of oxidative phosphorylation.
However, there is no simple procedure to measure ADPconcentration and thus the activity rates of and in a daily endurance performance analysis. For an application of the model as a tool of endurance performance testing, and must be calculated without measuring the free ADPconcentration. This is possible when the mentioned equations are transposed from ADP in depended equations.
Step 5: Transformation from ADP depended equations into depended equations
Equation 6: Calculation for the activity of oxidative phosphorylation ( ) as dictated by workload (P) and bodyweight.
Equation 7: Calculation of free ADPconcentration with respect to activated oxidative phosphorylation ( ) and maximal oxygen uptake ( ).
Equation 8: Calculation of glycolysis activity with respect to activated oxidative phosphorylation (VO_{2ss}) and maximal glycolytic rate ( ).
Furthermore, can also be calculated as demonstrated in Equation 5.
Step 6: Calculation of PMLSS_{C} depending on
Equation 9: Calculation in the activity of glycolysis with respect to the activation oxidative phosphorylation ( ) and maximal glycolytic rate ( ).
Equation 10: Calculation of power in MLSS (PMLSS_{C}) depending on the activity of oxidative phosphorylation ( ), bodyweight and oxygen/workloadconstant (Ks4).
Therefore the relation between and power expressed as Ks4 must be known. In the present paper Ks4 was set to a constant value of 11.7 O_{2}/W [3].
Constant load tests
Subjects performed at least two 30 min constant load exercise tests at a cadence of 70–80 rpm for determination the PMLSS_{E}[2]. The first constantload test according to PMLSS_{C} started after a warmup of 3 minutes at a power corresponding to 60% of the PMLSS_{C} rate. Blood samples were taken during rest, after 4 and 8 min, and at subsequent 2 min intervals until the end of the test. The PMLSS_{E} was defined as the highest workload that can be maintained without an increase of bloodlactateconcentration of more than 0.05 mmol·l^{−1}·min^{−1} during the last 20 minutes of the test. Depending on bloodlactateconcentration, power in the next constant load test was set higher or lower by 10 W.
Statistical analysis
All data were analyzed using the software SPSS version 14. Descriptive statistics were calculated from the data (means, standard deviations (SD), minimum and maximum values). Normal distribution was verified using the ShapiroWilkTest. Relationship between variables was investigated using orthogonal regression and correlation. The level of significance was set at α = 0.05 for all analyses.
Results
Results of maximum metabolic performance tests and calculated and experimental power in maximal lactatesteady state
Subject  (mmol·l^{−1}·s^{−1})  (ml·min^{−1}·kg^{−1})  Bodyweight (kg)  PMLSS_{C}(W)  PMLSS_{E} (W)  Difference PMLSS_{C} PMLSS_{E} (W) 

1  0.87  70.4  58.8  233  233  0 
2  0.67  69.7  70.65  294  244  50 
3  0.78  68.4  78.65  305  295  10 
4  0.89  64.8  70.00  246  266  −20 
5  1.39  61.0  66.2  182  172  10 
6  0.74  60.0  62.7  208  198  10 
7  1.02  55.8  76.65  207  187  20 
8  0.98  48.0  71.9  157  147  10 
9  0.98  56.6  80.1  224  204  20 
10  1.07  71.3  75.8  291  271  20 
11  0.74  47.1  78.55  184  144  40 
12  0.94  62.7  78.35  258  278  −20 
13  0.81  49.0  79.00  190  180  10 
± s  0.91 ± 0.18  60.4 ± 8.6  72.9 ± 6.8  229 ± 47  217 ± 51  12 ± 20 
min  1.39  71.3  80.1  305  295  50 
max  0.67  47.1  58.8  157  144  −20 
Discussion
The aim of the present investigation was to compare the calculated and experimentally determined power output in MLSS. The comparison of PMLSS_{C} and PMLSS_{E} showed a highly significant correlation (0.92), with only a mean difference of 12 W ± 20 W between the two methods. The results of the present paper accords to previous comparisons between the different lactateconcepts and MLSS. It is well known that different lactate threshold concepts approximate in average MLSS rather well. Van Schuylenbergh et al. [22] published highly significant correlations between MLSS and OBLA and the Dmax method (r = 0.94 and r = 0.89, respectively). Heck [4] also evaluated correlations between MLSS and OBLA and individual anaerobic threshold of r = 0.92 and r = 0.87, respectively. However, as already mentioned, the investigation of Hauser et al. [5] showed large individual differences comparing power of thresholdconcepts with power in MLSS. Therefore the calculation method is at least as useful the application of lactateconcepts to detect MLSS.
In contrast to lactateconcepts, however, by using the calculation method it is also possible to show the influence of individual and on MLSS, as well as their combined effects. This can be highlighted for subjects with similar values, for example subject 5 and 12 at 61.0 and 62.7 ml·min^{−1}·kg^{−1} respectively. Using the classical interpretation, endurance performance of these subjects would be nearly the same, yet interestingly PMLSS_{E} of subject 5 and 12 were completely different (172 vs. 278 W). To explain this difference of 106 W, it is not possible to use only , but differences in of both subjects (1.39 vs. 0.94 mmol·l^{−1}·s^{−1}) is also required. Therefore, subject 5 produces significantly more lactate within the muscle cell per second in contrast to subject 12. When related to the same , this higher lactate production rate leads to a reduction of MLSS [10, 12].
On the other hand it also seems pertinent to focus on subjects with the same PMLSS_{E,} for example subject 5 and 13 (172 vs. 180 W). It is essential to mention that and values of these subjects are completely different (61 vs. 49 ml·min^{−1}·kg^{−1} and 1.39 vs. 0.81 mmol·l^{−1}·s^{−1}, respectively). This particular example explains, why individuals with the same MLSS could originate by completely different combinations of and as previously suggested by Bleicher et al. [12]. Therefore the knowledge of and and the application of the calculation method could help for a better interpretation of MLSS.
Limitations
The reason for the overestimation of PMLSS_{C} is likely caused by methodological as well as physiological aspects related to its calculation. It is well known, that a high positive correlation between and PMLSS exists, which incidentally was confirmed in the present study, and highlights the importance of concerning PMLSS. The determination of is a valid test procedure and well established in performance and clinical diagnostics [23]. However, Mader and Heck [3], Bleicher et al. [12], Heck and Schulz [14] and Mader [11] showed that on a theoretical basis, must have a significant influence on PMLSS. In the present investigation shows no correlation with PMLSS_{E}, which was probably caused by the small range of values measured in this investigation. Furthermore, the missing correlation between and PMLSS_{E} as well as the overestimation of PMLSS_{C} may have been caused by the methodological procedure in determining the maximal anaerobic performance. For example, in the present study was measured by a sprinttest lasting 15 s. It is possible that testing by using a test duration lower than 15 s would lead to higher maximal glycolytic rates and therefore on the basis of the same to a lower PMLSS [3, 12, 14]. Hauser [24] showed, that increases by 8% when measured using a 13 s sprinttest compared to a 15 s sprinttest. If the present of 0.91 mmol·l^{−1}·s^{−1} would be increased by 8%, the PMLSS_{C} would have been 224 W. The bias between PMLSS_{C} and PMLSS_{E} would only be −7 W, which could from a practical point of view be neglected. Therefore test procedures of must receive greater focus in future investigations.
Another reason for the differences between the two methods could be the defined interval of 10 W between two constantload tests, which was used because of time and economic reasons. Using the interval of 10 W it is possible, that PMLSS_{E} is underestimated by a mean by 4  5 W. Consequently, it is possible that PMLSS_{E} does not represent the PMLSS exactly. The possible increase of PMLSS_{E} of 4  5 W would lead to a decrease in the difference between PMLSS_{C} and PMLSS_{E} of −7 W.
In addition, physiological reasons for differences could be based on the biological variability of the parameters and constants that were used in the calculation. As pointed out by Mader and Heck, the relation between and power output (Ks4) has an important influence on PMLSS [3]. Again according to Mader and Heck [3] Ks4 was set to 11.7 O_{2}/W in the present study. This relation corresponds exactly to the determined mean value of Ks4 used with the cycle ergometer. However, Ks4 varies on an interindividual basis [3], and only a theoretical increase of Ks4 by 2.5% would lead to a 224 W decrease in PMLSS_{C}. In addition, the daytoday variability of and also has important influences on PMLSS, with a mean withinsubject variation of 5.6% of leading to deviations in PMLSS_{C} of ± 30 W [25]. In contrast, the biological variability of still remains unknown.
Conclusion
The mathematical method introduced by Mader and Heck [3] for the determination of PMLSS represents an accurate method similar to that of previous lactatethreshold concepts. In contrast to lactatethreshold concepts, however, this novel calculation method is based on and that can be used for explaining the origin of PMLSS and therefore the metabolic response. The knowledge of both parameters, as well as their individual influence on MLSS, could be important for establishing training recommendations, which could lead to either an improvement in or by performing high intensity or low intensity exercise training, respectively.
Ethical standards
The experiments comply with the current laws of the country. The study was proved by Ethics Commission.
Notes
Abbreviations
 ADP:

Adenosine diphosphate
 AMP:

Adenosine monophosphate
 ATP:

Adenosine triphosphate
 AT:

Anaerobic threshold
 BLC:

Bloodlactateconcentration
 BW:

Body weight
 CLa_{rest} :

Bloodlacateconcentration during rest
 CP:

Crossing point
 Dmax method:

Lactate threshold concept
 IAT:

Individual anaerobic threshold
 Ks1:

50%activity constant of oxidative phosphorylation
 Ks2:

50%activity constant of glycolysis
 Ks4:

Oxygen/workload equivalent
 MaxPostLa:

Maximum post excercise blood lactate concentration
 MLSS:

Maximal lactatesteadystate
 MLSSc:

Calculated maximal lactate steadystate
 MLSS_{E} :

Experimental maximal lactat steadystate
 OBLA:

Onset of blood lactate accumulation
 PMLSS:

Power in maximal lactatesteadystate
 PMLSS_{C} :

Power in calculated maximal lactatesteadystate
 PMLSS_{E} :

Power in experimental maximal lactatesteadystate
 PFK:

Phosphofructokinase
 P_{max} :

Maximal power
 rpm:

Revolutions per minute
 RER:

Respiratory exchange ratio
 SD:

Standard deviation
 t_{alac} :

Alactic time intervall
Declarations
Acknowledgements
The authors would like to thank Steffi Hallbauer and Jörg Kersten for their assistance in the laboratory and Scott Bowen for their help.
Funding
The publication coast of this article were founded by the German Research Foundation/DFG (Geschäftszeichen INST 270/2191) and the Chemnitz University of Technology in the funding programme Open Access Publishing.
Authors’ Affiliations
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