Figure 6
Effect of key system parameter values on GLUT4 translocation. Curve 'a' shows half maximal concentration of insulin required to switch-on GLUT4 translocation. Curve 'b' shows half maximal concentration of insulin required to switch-off GLUT4 translocation. Arrow indicates parameter used in the simulation. A. Half maximal concentration of insulin required for GLUT4 translocation at different values of dissociation constant [Kd2] for binding of second molecule of insulin to phosphorylated insulin bound receptor. Simulated results indicate decreased insulin sensitivity when Kd2 increased. B. Half maximal concentration of insulin required for GLUT4 translocation at different values of dissociation constant [Kd3] for binding of phosphorylated IRS-1 to PI3K species. Simulated results indicate decreased insulin sensitivity when Kd3 increased. C. Half maximal concentration of insulin required for GLUT4 translocation at different values of Michaelis-Menten constant [Km2] for dephosphorylation of phosphorylated IRS1 by PTP1B. Simulated results indicate decreased insulin sensitivity when Michaelis-Menten constant [Km2] was decreased due to increased affinity with dephosphorylating enzyme.