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Table 6 Mutants designed by the second protocol (X-Pro substitutions)

From: Is it possible to stabilize a thermophilic protein further using sequences and structures of mesophilic proteins: a theoretical case study concerning DgAS

Mutants φ / ψ (°) Secondary structurea Domain ΔΔGfb ΔLSE
E36P −68.08/−43.76 H N −1.10 −0.0000352
A44P −68.62/−30.86 H N 3.21 −0.0002585
A70P −51.82/−37.08 H N −2.01 −0.0002950
D113P −62.44/−35.24 H A −1.00 0.0007323
R132P −63.46/155.45 C A 3.97 −0.0023640
N354P −67.63/−36.53 H A 0.41 0.0009455
A415P −61.10/−41.68 H B’ −1.55 −0.0000822
V438P −67.20/134.06 E B’ −0.50 0.0005602
I486P −72.15/−31.78 H A 5.03 0.0027557
V571P −66.70/128.68 E C −0.79 −0.0013040
D585P −74.44/147.85 C C −0.93 −0.0016631
T601P −73.84/123.41 C C −0.25 −0.0013239
V608P −56.44/−34.38 H C 0.77 0.0017565
G637P −46.17/129.60 T C −1.25 −0.0003485
  1. a. Letter H, E, C and T represent helix, sheet, coil and turn, respectively.
  2. b. kcalmol-1.