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Table 6 Mutants designed by the second protocol (X-Pro substitutions)

From: Is it possible to stabilize a thermophilic protein further using sequences and structures of mesophilic proteins: a theoretical case study concerning DgAS

Mutants

φ / ψ (°)

Secondary structurea

Domain

ΔΔGfb

ΔLSE

E36P

−68.08/−43.76

H

N

−1.10

−0.0000352

A44P

−68.62/−30.86

H

N

3.21

−0.0002585

A70P

−51.82/−37.08

H

N

−2.01

−0.0002950

D113P

−62.44/−35.24

H

A

−1.00

0.0007323

R132P

−63.46/155.45

C

A

3.97

−0.0023640

N354P

−67.63/−36.53

H

A

0.41

0.0009455

A415P

−61.10/−41.68

H

B’

−1.55

−0.0000822

V438P

−67.20/134.06

E

B’

−0.50

0.0005602

I486P

−72.15/−31.78

H

A

5.03

0.0027557

V571P

−66.70/128.68

E

C

−0.79

−0.0013040

D585P

−74.44/147.85

C

C

−0.93

−0.0016631

T601P

−73.84/123.41

C

C

−0.25

−0.0013239

V608P

−56.44/−34.38

H

C

0.77

0.0017565

G637P

−46.17/129.60

T

C

−1.25

−0.0003485

  1. a. Letter H, E, C and T represent helix, sheet, coil and turn, respectively.
  2. b. kcalmol-1.