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Figure 7 | Theoretical Biology and Medical Modelling

Figure 7

From: The design of a new truncated and engineered alpha1-antitrypsin based on theoretical studies: an antiprotease therapeutics for pulmonary diseases

Figure 7

PCR colony screening was performed using plasmid-specific primers (pGAP forward and 3'AOX) to determine positive clones. PCR screening of 10 Pichia pastoris strains transformed with pGAPZαA/α1AT plasmid. The presence of the specific α1AT −1600 bp PCR amplicon was detected by 1% agarose gel electrophoresis (ethidium bromide staining), confirming the insertion of pGAPZ αA/α1AT plasmid to yeast genome. Lane 1–10: positive colonies of α1AT1-5 constructs, V1-5: PCR positive control (pGAPZ αA plasmid) and X33: PCR negative control, M: molecular markers.

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