Figure 8
Expression analysis of constructed vectors containing different α1ATs sequences by SDS–PAGE and western-blot. (A) 72 h after induction, supernatants were electrophoresed in 12% SDS–PAGE and stained by Coomassie blue as follows: lane M, broad-range molecular weight marker; lanes 1, 2, 3, 4 and 5 supernatant samples of α1AT 1, 2, 3, 4 and 5, respectively. (B) Proteins were transferred onto a PVDF membrane and identified by immunoblotting using primary and secondary commercial antibodies as follows: lane M, schematic representation of the broad-range marker; lane 1, 2, 3, 4 and 5, supernatant of 72 h inducing culture of α1AT 1, 2, 3,4 and 5, respectively, which treated rabbit anti-AAT polyclonal antibody and then secondary Goat anti-rabbit polyclonal antibody.