Figure 9
Elastase inhibitory assay of the five α1AT structures. α1AT and modified derivatives were incubated with a natural target of α1AT and monitored over 20 min. The substrate is a BODIPY-FL-labeled DQ elastin conjugate that is highly labeled so that the fluorescence signal is quenched until enzymatic digestion yields highly fluorescent fragments. Since DQ elastin is digested by elastase, the inhibition of elastase activity by α1AT can be determined.