Figure 2From: Mathematically optimized cryoprotectant equilibration procedures for cryopreservation of human oocytesComparison of mathematically optimized protocols for removal of 6Â mol/L EG from human oocytes. The red line shows results from our previous study [16], which involved piecewise linear parameterization of m1(Ï„) and m2(Ï„) and a goal state fixed at the isotonic cell volume. The orange line shows results for the same piecewise linear parameterization of m1(Ï„) and m2(Ï„), but with a goal state that was not fixed at a specified final volume. The green and blue lines show two-step and three-step piecewise constant procedures, which had goal states that were not fixed at a specific final volume. The horizontal dotted lines in the top figure show the osmotic tolerance limits. Note that the nondimensional non-permeating solute concentration m1 can be converted to molal units by multiplying by 0.3Â Osm/kg.Back to article page