Comparison of mathematically optimized protocols for removal of 6 mol/L EG from human oocytes. The red line shows results from our previous study , which involved piecewise linear parameterization of m1(τ) and m2(τ) and a goal state fixed at the isotonic cell volume. The orange line shows results for the same piecewise linear parameterization of m1(τ) and m2(τ), but with a goal state that was not fixed at a specified final volume. The green and blue lines show two-step and three-step piecewise constant procedures, which had goal states that were not fixed at a specific final volume. The horizontal dotted lines in the top figure show the osmotic tolerance limits. Note that the nondimensional non-permeating solute concentration m1 can be converted to molal units by multiplying by 0.3 Osm/kg.