Figure 5

Addition and removal of 8.5 mol/L EG using the methods indicated with asterisks in Table2. The intracellular EG concentration (in mol/L) and transmembrane fluxes of water and EG are illustrated at several points in the CPA addition and removal process. During EG addition, the extracellular EG concentration was equal to 1.3 mol/L, 2.1 mol/L and 8.5 mol/L during steps 1, 2 and 3, respectively. The horizontal dotted lines show the osmotic tolerance limits.