|
Procedure
|
Step
|
Non-permeating solute, M1(Osm/kg)
|
EG, M2(Osm/kg)
|
Time, t(min)
|
Toxicity (Jα)
|
|---|
|
Constraints:
|
0 ≤ M
1
≤ 24
|
0 ≤ M
2
≤ 24
|
t ≥ 0
| |
|
Addition
|
1
|
0
|
1.4
|
20
|
130
|
| |
2
|
0
|
2.4
|
16
|
| |
3
|
0
|
24
|
0.094
|
|
Removal
|
1
|
1.8
|
0
|
3.6
|
22
|
| |
2
|
0.66
|
0
|
15
|
|
Constraints:
|
0 ≤ M
1
≤ 24
|
0 ≤ M
2
≤ 24
|
t ≥ 1
| |
|
Addition
|
1
|
0
|
1.3
|
19
|
240
|
| |
2
|
0
|
2.4
|
16
|
| |
3
|
0
|
17
|
1.0
|
|
Removal
|
1
|
2.0
|
0
|
3.6
|
25
|
| |
2
|
0.65
|
0
|
15
|
|
Constraints:
|
0 ≤ M
1
≤ 24
|
0 ≤ M
2
≤ 16
|
t ≥ 1
| |
|
Addition
|
1
|
0
|
1.4
|
20
|
250
|
| |
2
|
0
|
2.5
|
16
|
| |
3
|
1.2
|
16
|
1.0
|
|
Removal
|
1
|
1.8
|
0
|
3.5
|
22
|
| |
2
|
0.68
|
0
|
13
|
|
Constraints:
|
0.05 ≤ M
1
≤ 24
|
0 ≤ M
2
≤ 16
|
t ≥ 1
| |
|
Addition*
|
1
|
0.050
|
1.4
|
24
| |
| |
2
|
0.050
|
2.4
|
20
|
280
|
| |
3
|
1.2
|
16
|
1.0
| |
|
Removal*
|
1
|
1.8
|
0
|
3.6
|
23
|
| |
2
|
0.66
|
0
|
14
| |
- The goal state for EG addition was sf/wf = 53.7, which is equivalent to an intracellular concentration of 8.5 mol/L, or 16 Osm/kg.
- *These procedures are illustrated in Figure 5.
