Figure 4

Statistical summary of the aged cell analysis based on Flp-out clone induction. (A-B) Induction of Flp-Out clones in the Drosophila wing disc epithelium produces cell populations marked with GFP (green). Discs Large (DLG; red) marks the septate junctions. (A) Sub-panels i-vi show examples of single cell clones (SCC’s). White stars mark neighboring cells; the labeled cell’s polygonal topology is designated in white. (B) Sub-panels i-vi show examples of two-cell clones (TCC’s). White stars mark neighboring cells; labeled cells’ polygonal topologies are designated in white. (C) The average mitotic cell has approximately seven sides [24], whereas the average non-mitotic cell has approximately six sides [12]. These differences are significant (p < 10^-106; ttest2 in Matlab). Stars represent statistical significance. Sample sizes for the empirical overall and mitotic distributions are given under the heading “Sample sizes for overall and mitotic cell shape distributions” in the Methods section. (D) The average single-cell clone has approximately 6.66 sides, whereas the average two-cell clone has approximately 6.09 sides. These differences are significant (p < 10^-8; ttest2 in Matlab). Stars represent statistical significance. Sample sizes for the SCC and TCC distributions are given under the heading “Sample sizes for single cell clone (SCC) analysis and two cell clone (TCC) analysis” in the Methods section. (E) The mitotic cell shape distribution (black) is approximately an integer shift of the overall cell shape distribution (grey). (F) The single cell clone (SCC) distribution (black) is shifted relative to the two-cell clone (TCC) distribution (grey). Panels (E) and (F) display the same data as panels (C) and (D), respectively.