Figure 4
The V-D-J junctional diversity created by P region, exonuclease trimming and N region addition significantly contributes to a large VH CDR3 repertoire. A The numbers of N, P nucleotides added at the V-D-J junctions and nucleotides deleted by exonuclease trimming are shown, with the mean ± SD. The p values were determined using ANOVA. All statistically significant differences are indicated. * = p < 0.05, ** = p < 0.01, *** = p < 0.001. B The numbers of N1, N2, P3'V, P5'D and P5'J nucleotides added at the V-D-J junctions and nucleotides deleted at the 3'V, 5'D, 3'D and 5'J by exonuclease trimming are shown, with the mean ± SD. The p values were determined using ANOVA. All statistically significant differences are indicated. * = p < 0.05, ** = p < 0.01, *** = p < 0.001.