Figure 10

Two images of the same specimen with different alignment of the filament and condenser. (a) There is a non-uniform illumination problem for the first image as illustrated in Figure 12(a). L* values change throughout the image regarding the non-stained white areas; (b) The second image has some uniform illumination problem as illustrated in Figure 12(b), even though the alignment of the filament and condenser is better and the calibration of the microscope that is a good quality has been performed recently. The L* values in four quadrants of the image (as it is divided in four equal parts) regarding the non-stained white areas are almost equal to each other, but, smaller than 100 that is the desired value for the non-stained sections.