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Table 1 Summary of predicted tyrosine phosphorylation sites.

From: Tyrosine phosphorylation of myosin heavy chain during skeletal muscle differentiation: an integrated bioinformatics approach

Sitea

NetPhosb

Scansitec

2Dd

Surface Accessibilitye

 

MYH1

MYH4

MYH2

MYH8

MYSS_CHICK

MYH3_CHICK

MYH3

MYH1

MYH4

MYH2

MYH8

MYSS_CHICK

MYH3_CHICK

MYH3

MYH1

1BR2

1B7T:A

2MYS: A

Mean

47

-

-

-

Y

   

-

-

-

    

E(4)

(14.5)

(21)

(16)

18.5

54

-

-

-

-

-

-

Y

-

-

-

-

-

-

 

E(7)

 

(52)

(60)

56.0

85

Y

Y

Y

Y

Y

Y

Y

       

C(2)

(34.7)

(28)

16

26.2

163

Y

Y

Y

Y

Y

Y

Y

YP

YP

YP

YP

YP

YP

YP

H(9)

13.3

47

8

22.8

286

Y

Y

Y

Y

Y

Y

Y

  

Y

Y

   

H(7)

(0)

0

0

0.0

313

Y

-

     

YP

-

Y

Y

YP

Y

Y

C(4)

7

5

8

6.7

413

Y

Y

Y

Y

-

Y

Y

    

-

  

E(7)

  

(68)

68.0

435

     

-

Y

     

-

 

H(9)

(0.7)

4

3

2.6

504

Y

Y

Y

Y

Y

Y

Y

       

H(9)

0

6

9

5.0

719

Y

Y

Y

Y

Y

  

YP

YP

YP

YP

YP

YP

Y

H(6)

(21.8)

19

9

16.6

1379

Y

Y

Y

Y

Y

Y

Y

       

H(6)

    

1464

-

Y

-

Y

Y

Y

-

-

 

-

   

-

H(7)

    

1492

Y

Y

Y

Y

Y

Y

Y

   

YP

  

P

H(6)

    

1856

Y

Y

Y

Y

Y

Y

Y

Y

Y

Y

Y

Y

Y

Y

H(4)

    
  1. a. Sites are numbered relative to the MHC sequence MYH1_HUMAN/P12882.
  2. b. Y indicates predicted tyrosine phosphorylation site in all the sequences of orthologous group, with a score of ≥ 0.8 in at least one human sequence. Dashes indicate lack of a tyrosine in that position.
  3. c. Y indicates predicted tyrosine phosphorylation site in all the sequences of orthologous group on 'Low Stringency'. P indicates predicted phosphotyrosine recognition site in all the sequences of orthologous group on 'Low Stringency'. Dashes indicate lack of a tyrosine in that position.
  4. d. PSIPRED (McGuffin, Bryson and Jones 2000) secondary structure position for MYH1_HUMAN. Letters indicate predicted secondary structure (H = helix, E = strand, C = coil). Numbers in brackets are confidence measures (0 = low, 9 = high).
  5. e. Surface accessibility figures are "numbers of water molecules in contact with this residue *10, or residue water exposed surface in Angstrom**2" (Kabsch and Sander 1983). Missing values indicate residues missing from the PDB structure. Values in brackets indicate residues that are not tyrosines in the PDB structure.