Figure 2

Hypothetical pathways for simulation experiments. Simple pathway models employed to evaluate the accuracy of the hybrid method in comparison with conventional kinetic simulation. The reactions in the boxes were replaced with a static module in the hybrid models. (a) A pathway model with an over-determined static module. (b) A model including an underdetermined static module. (c) A simple linear pathway model. (d) A pathway map of the glycolysis model [13, 20]. See Tables 4 and 5 in Additional file 1 for the abbreviations of the metabolites and the enzymes, respectively.