Kinetic modeling of tricarboxylic acid cycle and glyoxylate bypass in Mycobacterium tuberculosis, and its application to assessment of drug targets

Table 1 Metabolites of the models with known concentrations (with references indicated in square brackets)

Escherichia coli			Mycobacterium tuberculosis
Metabolite	Concentration in glucose condition (in mM)	Concentration in acetate condition (in mM)	Metabolite	Concentration (in mM)
acetyl-CoA	0.5 [16]	0.5 [16]	succinate	2.464 (derived from Tian et. al [13])
citrate	3 [16]	9 [16]	fumarate	0.08528 (derived from Tian et. al [13])
iso citrate	0.018^a [16]	0.15 [16]	malate	0.408 (derived from Tian et. al [13])
succinate	0.6 [16]	6 [16]	oxaloacetate	0.0003 (assumed)
malate	1.8 [16]	5 [16]	CoA	0.0001 (assumed)
oxaloacetate	0.004^b	0.0014 (assumed)
CoA	0.0001 (assumed)	0.0001 (assumed)

^aIsocitrate concentration was inferred from a graph shown by Walsh et. al [16]. The value in the graph was 0.025 mM at 30 minutes after addition of glucose to the medium, but it had a negative slope, so, a value of 0.018 mM was taken.
^bTaken as 2.4 times the concentration of oxaloacetate under growth on acetate because flux leading to the synthesis of oxaloacetate under growth on glucose is 2.4 times of that under growth on acetate [15].

ISSN: 1742-4682