Input of experimental images into modeling software. (A) Computer-assisted tracing of anatomical boundaries relevant to diaphragm development in an E11.5 mouse (black – body wall, red – aorta, yellow – esophageal mesentery, blue – PPF, green – lungs). Where the relevant structures are not captured on a single section, these boundaries represent a composite orthographic projection of serial sections (see Fig. 5 and text). (B) Digital capture of coordinate-space data points along anatomical boundaries using GetData© 2.17 software. (C) Regeneration of digitized anatomical boundaries by the Nudge++™ modeling software (green – body wall, red – aorta, yellow – esophageal mesentery, white – PPF). (D) Nudge++ image with the PPF populated by model cells. Cells are not added to the posterolateral aspect of the left posterior PPF defect (arrow) to recreate experimental findings in the nitrofen model (see text and Fig. 6).