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Table 1 Kinetic parameters of Escherichia coli adenylosuccinate synthetase. Our model output, and data provided from the literature.

From: A mathematical model for the adenylosuccinate synthetase reaction involved in purine biosynthesis

Parameter Model output Literature data Reference
Km GTP 0.023 mM 0.01 mM 7
   0.048 mM 6
   0.0221 mM 10
   0.0262 ± 0.0023 mM 12
Km IMP 0.02 mM 0.02 mM 7
   0.054 mM 6
   0.0243 mM 10
   0.0278 ± 0.0013 mM 12
Km ASP 0.3 mM 0.35 mM 7
   0.225 ± 0.016 mM 9
   0.27 mM 6
   0.191 mM 10
   0.23 ± 0.04 mM 12
Ki SUCC * 8 mM - -
Ki GDP 0.008 mM 0.022 mM 6
Ki ASUC 0.0075 mM 0.27 mM (for IMP)** 6
   0.47 mM (for ASP) 6
Km Mg 0.08 mM 0.114 ± 0.026 mM 9
Ki GMP 0.024 mM 0.092 mM (for GTP) 6
   0.074 mM (for IMP) 6
Ki AMP 0.01 mM 0.095 mM 6
  1. * The effect of SUCC was examined by Rudolph and Fromm [7]. However, their formula is too complicated (it describes the effect of one inhibitor using four different constants) and the inhibition constants were not evaluated.
  2. ** In 1963 Wyngaarden and Greenland [6] proposed that ASUC is a competitive inhibitor of both IMP and ASP, and GMP is a competitive inhibitor of both IMP and GTP.