Figure 2

DNA Distribution in the cell nucleus. Distribution of DNA in HeLa cells; DNA is visualised after incorporation of 10 μM BrdU for 24 h. Cells were then processed for electron microscopy and the Br-DNA visualized after denaturation of DNA (1 M HCl, 30 min). DNA is distributed in a sponge-like pattern; this distribution is more obvious in panel B. Panel B shows a threshold image of DNA distribution. This image is very similar to the images obtained by viscoelastic phase separation [23]. Bar 500 nm.