Validation and quantification for optimizing parameters. (A) Current transfer to the root in artificially constructed dendritic tree shown in Figure 4D with tapering diameters. (B) Current transfer in reconstructed HSS dendritic tree assuming constant (2.3 μm) diameter for all dendritic branches (the maximal electrotonic distance in this case was similar to that of the HSS model with tapering dendritic diameter). (C) Current transfer in reconstructed dendrite, in which dendritic topology only minimized the total wiring from root to all points shown in Figure 4C but with tapering dendritic tree. (compare these graphs with Figure 1A, all having the same colour scale). In all cases the axon of the HSS model was appended to the artificially reconstructed dendrites. (D) Distributions of normalized current transfer in the different nodes of the dendrites of the real HSS model (black) and the three reconstructed dendrites (A – grey, B – red, C – blue).