Figure 2

Concept diagram for m × n microsphere matrix. A. Each circle in this diagram represents a set of microspheres (i.e. an SMPCS). Each of the superset identical groups (i.e. SMPCS-IDG) (m = 5) of are coated at different sensitivities. The SMPCS-IDG's of m are across the top, labeled 01–05. Note that now each m is a superset composed of 5 microsphere set identifiers (i.e. an SMPCS-IDG). Each of n (01 to 05 for SMPCS-IDG 01, 06 to 10 for SMPCS-IDG 02, ...) microspheres that make up the superset SMPCS-IDG for m is coated in the same batch for identical sensitivity. Like figure 1, the m SMPCS-IDG's have serial dilutions (or some other useful difference in sensitivity method) in their manufacture. B. Processing of the intraplex using a simulated example. Step 1: On left is an m = 5 × n = 5 fluorescent reporter reading dataset graph for all SMPCS's, 01–25. (Note the outlier at 05, S5 that was removed for the set of n for the m SMPCS-IDG number 01.) Completion of step 1 is removal of outliers. Step 2: The result of this step is m averages, (means of n) using as input the n microsphere set fluorescence readings for each SMPCS-IDG. This is shown in the table. Each of these 5 means of n are averaged together to give a single mean of m. Step 3: Internal self mean ratios using the mean of m as denominator for each of the means of n from step 2. This is done in the same way as for the simple intraplex of figure 1.