Coordination of the dynamics of yeast sphingolipid metabolism during the diauxic shift

Table 3 Fold changes in mRNA's of model enzymes

Gene	ORF's	9 hr	11 hr	13 hr	15 hr	17 hr	19 hr	21 hr
		Fold Increases in mRNA (DeRisi *et al* ., 1997)
LCB4	YOR171C	1.1	1.16	1.3	0.92	1.02	1.61	1.37
LCB5	YLR260W	0.88	0.81	0.97	0.9	0.77	2.13	1.35
DPP1	YDR284C	0.93	0.91	1.67	1.15	0.98	1.54	0.83
LPP1	YDR503C	1.08	1.04	1.47	1.05	1.15	1.18	1.27
GPT2	YKR067W	1.01	1.05	1.16	1.35	1.64	2.10	2.36
GAT2/SCT1	YBL011W	0.96	0.94	1.09	0.83	0.85	1.15	0.72
FAS1	YKL182W	1.11	1.08	0.97	0.83	0.78	0.88	0.77
FAS2	YPL231W	0.95	1.03	0.95	0.92	0.71	0.71	0.71
LCB1	YMR296C	0.97	1	1.16	0.82	0.90	1.04	1.19
LCB2/SCS1	YDR062W	0.96	1.04	1.14	0.93	0.83	0.45	0.40
ELO1	YJL196C	0.95	0.85	1.23	1.09	1.33	0.79	0.41
ELO2/FEN1	YCR034W	1.09	1.11	1.89	1.01	0.75	0.54	0.32
ELO3/SUR4	YLR372W	1.06	1.27	1.56	0.95	1.05	0.43	0.23
		Fold Increases normalized against 11 hr values
LCB4	YOR171C	0.94	1	1.11	0.79	0.88	1.40	1.19
LCB5	YLR260W	1.08	1	1.20	1.11	0.95	2.65	1.66
DPP1	YDR284C	1.02	1	1.84	1.26	1.08	1.69	0.91
LPP1	YDR503C	1.03	1	1.41	1.01	1.10	1.13	1.22
GPT2	YKR067W	0.97	1	1.11	1.29	1.57	2.00	2.25
GAT2/SCT1	YBL011W	1.03	1	1.15	0.88	0.90	1.22	0.77
FAS1	YKL182W	1.04	1	0.90	0.77	0.72	0.82	0.72
FAS2	YPL231W	0.93	1	0.93	0.89	0.69	0.69	0.70
LCB1	YMR296C	0.98	1	1.16	0.82	0.91	1.05	1.20
LCB2/SCS1	YDR062W	0.93	1	1.09	0.90	0.80	0.44	0.39
ELO1	YJL196C	1.11	1	1.45	1.27	1.57	0.92	0.48
ELO2/FEN1	YCR034W	0.99	1	1.69	0.91	0.67	0.49	0.29
ELO3/SUR4	YLR372W	0.84	1	1.22	0.75	0.83	0.34	0.18

mRNA fold changes and corresponding values of enzyme activities in the model at the different time point, normalized against 11 hr data. Data from DeRisi et al. [1]. LCB4 – LCB5 and DPP1-LPP1 are a pair of enzymes with similar substrates and/or products and they represent the sphingoid base kinase (X₃₆) and the phosphatidate phosphatase (X₃₉), respectively. FAS1- FAS2 and LCB1-LCB2 correspond to sub-units for fatty acid synthase (X₅₂) and serine palmitoyl transferase (X₅₇), respectively. The three ELO's represent the battery of enzymes involved in fatty acid elongation represented in the model by Elo1p (X₅₉).

ISSN: 1742-4682