Figure 40

Protein monitoring by SHARPs. Specific and high affinity reacting proteins (SHARPs, 1–7) are designed and produced against a protein. The protein has 3 domains (A, B, C) and two main conformations (I, II). Domain B is exposed on the surface in conformation I and that form is detected by SHARPs 3–5. Domain B is sandwiched between domains A and C in configuration II and therefore this form is detected by SHARPs 1–2 and 6–7. A concentration and configuration dependent response is detected by a slot corresponding to the protein by the SHARPs CHIP (dots).