Figure 7

Changes at the molecular level for Cell No 48 with an increasing extrinsic EGF concentration (rule A), at an EGFR concentration of 800 nM. Three simulation runs are depicted where (from left to right) the EGF concentration increases from 2.65 × 1.0 to 2.65 × 5.9 and 2.65 × 6.0 nM. (From top to bottom) plotted are the absolute change of PLCγ, rate of change of PLCγ, and rate of change of ERK. Note that a phase transition emerges again between the EGF concentrations of 2.65 × 5.9 and 2.65 × 6.0 nM, hence at a lower concentration compared to the one depicted in Fig. 6 (EGFR concentration of 80 nM). In the two simulations around the phase transition, the maximum rates of change for both PLCγ and ERK (i.e., ROC_PLC and ROC_ERK at 2.65 × 5.9 and at 2.65 × 6.0 nM) are lower compared with those in Fig. 6 (i.e., ROC_PLC and ROC_ERK at 2.65 × 31.1 and at 2.65 × 31.2 nM). (For phenotype labeling see Fig. 4).