Gut ABM with %Isch = 35 at 4 and 18 h. Figure 11a is a graph that demonstrates the timecourses of Cellwall Occludin and Cytoplasmic Occludin over 18 hrs with an initial "%Isch" = 35. The Cellwall Occludin is shown in Red, the Cytoplasm Occludin is shown in Blue. Note that Cytoplasm Occludin has started to recover at ~12 h. The delay in recovery of the Cellwall Occludin is due to the persistent effect of NO on Occludin localization via the NSF pathway. Figure 11b is the view of gut ABM from the epithelial surface at 4 hours. Note the darkened sections of the epithelial surface, denoting impaired TJ protein metabolism and localization. The areas near the center and right upper corner of the layer show the change in shape from "cube" to "cone," indicative of TJ failure. Figure 11c is the view of gut ABM from the epithelial surface at 18 hours. Note how there has been progression to generalized TJ barrier failure, with only the small area in the left lower corner still with some intact TJs.