Figure 4

Screen shot of the Graphical User Interface of the EBABM. Control buttons are on the Left; Graphical Output of the simulation is in the center. Graphs of variables corresponding to levels of mediators and tight junction proteins are at the bottom and right. In the Graphical Output Caco-2 agents are seen as pink squares, those with intact Tight Junctions bordered in yellow (Letter A), those with failed Tight Junctions bordered in black (Letter B). This particular run is with the addition of cytomix (Letter C), seen after 12 hours of incubation (Letter D). The heterogeneous pattern of tight junction failure can be seen in the Graphical Output. Levels of Caco-2 iNOS activation can be seen in Graph Letter E, and produced Nitric Oxide (NO) can be seen in Graph Letter F. Of note, the total amount of tight junction protein occludin does decrease slightly (Graph Letter G), but the amount of occludin localized in the cell membrane drops much more rapidly (Graph Letter H), reflecting the impairment of occludin transport due to NO interference with NSF and subsequent loss of tight junction integrity.