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Table 1 REase (DNase) enzymes cutting HSV-2 genome in > 100, but < 400 sites

From: Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: Inherent potential for biosynthetic versus live recombinant microbicides

Enzyme name Genomic splices (palindrome)
AccBSI 164(gagcgg)
AccI 111(gt/mkac)
AclWI 225(ggatc)
AflIII 127(a/crygt)
Alw21I 203(gwgcw/c)
Alw26I 308 (gtctc)
AlwI 225 (ggatc)
ApaI 267 (gggcc/c)
AspHI 203 (gwgcw/c)
BbeI 261 (ggcgc/c)
Bbv12I 203 (gwgcw/c)
BsaWI 131 (w/ccggw)
Bse1I 155 (actgg)
BseNI 155 (actgg)
BsePI 349 (g/cgcgc)
BseRI 213 (gaggag)
BsiHKAI 203 (gwgcw/c)
BsiI 111 (ctcgtg)
BsmAI 308 (gtctc)
BsmBI 149 (cgtctc)
Bsp120I 267 (g/ggccc)
BspMI 123 (acctgc)
BpmI 170 (ctggag)
BsaAI 155 (yac/gtr)
BsrBI 164 (gagcgg)
BsrI 155 (actgg
BsrSI 155 (actgg)
BssHII 349 (g/cgcgc)
BssSI 111 (ctcgtg)
BstZI 338 (c/ggccg)
BssT1I 124 (c/cwwgg)
BstD102I 164 (gagcgg)
BstDEI 139 (c/tnag)
BstF5I 292 (ggatg)
BstX2I 108 (r/gatcy)
BstYI 108 (r/gatcy)
Cfr9I 286 (c/ccggg)
DdeI 139 (c/tnag)
EagI 338 (c/ggccg)
EclXI 338 (c/ggccg)
Eco130I 124(c/cwwgg)
EcoT14I 124 (c/cwwgg)
EheI 261 (ggc/gcc)
ErhI 124 (c/cwwgg)
Esp3I 149 (cgtctc)
FokI 292 (ggatg)
HincII 105 (gty/rac)
HindII 105 (gty/rac)
HinfI 318 (g/antc)
HphI 280 (ggtga)
KasI 261 (g/gcgcc)
MaeIII 244 (/gtnac)
MboII 261 (gaaga)
MflI 108 (r/gatcy)
MroNI 250 (g/ccggc)
MseI 116 (t/taa)
MslI 124(caynn/nnrtg)
NaeI 250 (gcc/ggc)
NarI 261 (gg/cgcc)
NgoAIV 250 (g/ccggc)
NgoMI 250 (g/ccggc)
NspI 104 (rcatg/y)
PleI 212 (gagtc)
PpuMI 169 (rg/gwccy)
Psp5II 169 (rg/gwccy)
PspAI 286 (c/ccggg)
PspALI 286 (ccc/ggg)
PspOMI 267 (g/ggccc)
SfaNI 279 (gcatc)
SmaI 286 (ccc/ggg)
TfiI 106 (g/awtc)
Tru1I 116 (t/taa)
Tru9I 116 (t/taa)
Tsp45I 184 (/gtsac)
TspRI 109 (cagtg)
XhoII 108 (r/gatcy)
XmaI 286 (c/ccggg)
XmaIII 338 (c/ggccg)
  1. 77 total enzymes