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Table 3 REase enzymes cutting HSV-2 genome in 700 or more times

From: Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: Inherent potential for biosynthetic versus live recombinant microbicides

Enzyme name

Genomic splices (palindrome)

1BmyI*

773 (gdgch/c)

2Bsp1286I*+#

773 (gdgch/c)

3Bst2UI*+

824 (cc/wgg)

4BstNI*+#

824 (cc/wgg)

5BstOI*+

824 (cc/wgg)

6EcoRII*+#

824 (/ccwgg)

7HgaI*+#

831 (gacgc)

8MvaI*+#

824 (cc/wgg)

9SduI*+#

773 (gdgch/c)

  1. *Type II restriction enzyme subtype: P; +commercially available; #Enzyme gene cloned 1–9 Source of REase: Bacillus mycoides [21], Bacillus sphaericus [22], Bacillus stearothermophilus 2U, Bacillus stearothermophilus [23], Bacillus stearothermophilus O22, Escherichia coli R245 [24], Haemophilus gallinarum [25] Micrococcus varians RFL19 [26] and Streptococcus durans RFL3[27]