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Table 4 Physiochemical parameters of EcoRII as predicted from the amino acid sequence alignments

From: Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: Inherent potential for biosynthetic versus live recombinant microbicides

Physicochemical parameter Value
Number of amino acids: 404
Molecular Weight 45611
Theoretical PI 6.10
Total number of negatively charged residues (Asp+Glu) 52
Total number of positive residues (Arg+Lys) 43
Atomic composition:  
   • Carbon(C) 2053
   • Hydrogen(H) 3205
   • Nitroge(N) 565
   • Oxygen(O) 393
   • Sulfur 10
Total number of atoms: 6426
Formula: C 2053 H 3205 N 565 O 593 S 10
Extinction coefficients: 47120(46870)
Estimated half-life(hours)  
   • (mammalian reticulocytes, in vitro) 30 hour
   • (yeast, in vivo) > 20 hours
   • (Escherichia coli, in vivo) > 10 hours
Instability index: 45.04
Aliphatic index: 97.05
Grand average of hydropathicity (GRAVY) -0.183