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Table 4 Physiochemical parameters of EcoRII as predicted from the amino acid sequence alignments

From: Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: Inherent potential for biosynthetic versus live recombinant microbicides

Physicochemical parameter

Value

Number of amino acids:

404

Molecular Weight

45611

Theoretical PI

6.10

Total number of negatively charged residues (Asp+Glu)

52

Total number of positive residues (Arg+Lys)

43

Atomic composition:

 

   • Carbon(C)

2053

   • Hydrogen(H)

3205

   • Nitroge(N)

565

   • Oxygen(O)

393

   • Sulfur

10

Total number of atoms:

6426

Formula:

C 2053 H 3205 N 565 O 593 S 10

Extinction coefficients:

47120(46870)

Estimated half-life(hours)

 

   • (mammalian reticulocytes, in vitro)

30 hour

   • (yeast, in vivo)

> 20 hours

   • (Escherichia coli, in vivo)

> 10 hours

Instability index:

45.04

Aliphatic index:

97.05

Grand average of hydropathicity (GRAVY)

-0.183