A: Codon usage analysis of wild type and optimized gene for expression in plants. The value of 100 is set for the codon with the highest usage frequency for a given amino acid in the desired expression into plants. This procedure allows us to compare the adaptiveness of different codons relative to each other (relative adaptiveness). Plots represent the relative adaptiveness of a given codon at the indicated codon position. B: GC analysis of wild type and optimized chimeric gene. Plots represent the average GC content, before and after optimization.