From: In silico analysis of chimeric espA, eae and tir fragments of Escherichia coli O157:H7 for oral immunogenic applications
Splice site
Original
Optimized
GGTAAG
2
0
GGTGAT
4
GTAAAA
1
GTAAGT
GTACGT
Poly A
AATAAA
AATGAA
AATGGA
TATAAA
AATAAT
3
AAAAAAA
Poly T
TTTTTT
Destabilizing element
ATTTA