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Figure 4 | Theoretical Biology and Medical Modelling

Figure 4

From: Computational investigation of epithelial cell dynamic phenotype in vitro

Figure 4

Cyst growth in simulated and in vitro MDCK cell culture. (A) MDCK cells grown in 3D matrix form lumen-enclosing cystic organoids surrounded by a layer of polarized cells. Cells composing the cysts maintain three surface types: apical (green), basal and lateral (red). Note the roundish contour typical of MDCK cysts. For growth and staining details, see [11]. Bar: 10 μm. (B) ISEA1 CELLS in EMBEDDED condition produced stable, cystic structures enclosing LUMINAL SPACE; all CELLS were POLARIZED (red). Many CYSTS like the one shown, had irregular, non-convex shapes unlike their in vitro counterpart. (C) ISEA2 CELLS under the same condition also developed stable CYSTS; almost all stabilized CYSTS had convex shapes. Note that a hexagonal CYST within the hexagonally discretized space maps to a roundish cross-section through a MDCK cyst in vitro. (D) ISEA2 CELLS formed CYSTS that tended to be smaller than those of ISEA1 (average 27 vs 31 CELLS per CYST). The CELL count represents mean values after 50 simulation cycles of 100 Monte Carlo runs.

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