Canonical reaction topology underlying TLR4-mediated signaling events. This canonical topology was assembled according to well-documented studies on the reaction steps deployed during TLR4-mediated signaling in macrophages, in response to LPS stimulation. Our kinetic model accounts for the reaction dynamics of 76 molecular species, including single species and transiently-formed complexes resulting from the aggregation of two or more species. Some intermediate species are not illustrated; only key reaction components are shown. Our kinetic modeling approach is founded on basic principles of biochemical reaction, accounted for via simple mass action law (both first and second order kinetics) and generalizations of Michaelis-Menten reaction kinetics.