Figure 1

Schematics of HSV-II genome- cleavage by ZFN. This figure graphically illustrates the sites and frequency of ZFN cleavage of the HSV-2 genome by the 684 ZFNs identified. Cleavage was present at almost all positions within the HSV-2 genome. Note, however, that the highest incidence of HSV-2 genomic cleavage is situated within the last 30,950 base-pairs (154,784/5: ~between the genomic context coordinates 0.80 and 1.00) at the 3' end of the HSV-2 genome. That said, at approximately 3,094.9 bp (30,950/10) before and after the 5' and 3' ends of the HSV-2 genome (genomic context coordinates 0.02 and 0.98, respectively), there is another array of specificity sites for ZFNs that are potentially best suited for the complete excision of over 60% of HSV-2 genomic material from within infected human cells.