Figure 15

GMABM response to transient intestinal ischemia. The effects of 30 minutes of transient intestinal ischemia, including release of dynorphin and adenosine into the intestinal lumen, were simulated. We utilized two in silico experimental groups: a control group (Pseudomonas agents = 0) and a Pseudomonas group (Pseudomonas agents = 10). Transient ischemia alone produced no significant disruption (Control). The combination of simulated transient intestinal ischemia and the presence of Pseudomonas agents yielded a 40% decrease in barrier function at 24 hours of simulation time. Note that the initiation of the effect can be seen between 6 and 12 hours, suggesting that this period should be targeted for sampling in any future in vivo experiments to obtain the most potentially relevant data.