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Table 3 Biochemical Parameters ( μ M, μ M/hr,/hr)

From: The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model

Reaction Parameter Model value Literature value References  
V CYP      
  p 20    
  d 18000    
  n 2    
V CYP1A2 cytochrome CYP1A2     
  K m 3430 3430-3440 [16]  
  V max 0.55    
V CYP2E1 cytochrome CYP2E1     
  K m 677 677-1260 [16]  
  V max 345    
V CYP3A4 cytochrome CYP3A4     
  K m 276 276-313 [16]  
  V max 0.99    
V (l/t)SULT sulfation     
  K m apap 97 97 [24]  
  K m paps 5.6 5.6 [25]  
  V max in liver 1785    
  V max in tissue 357    
PAPS metabolism      
  V lpaps (synthesis liver) 0.1    
  k lpaps (linear removal) 0.0033    
  V tpaps (synthesis tissue) 0.01    
  k tpaps (linear removal) 0.00033    
V GST glutathione transferase     
  K m GSH 5200 4600–5600 [21, 22]  
  K m NAPQI 15    
  V max 72000    
covalent binding      
  k bind 1000    
  k rev 0.25    
V(l/t)UGT=VUGT1 + VUGT2 + VUGT3 + VUGT4      
V UGT1 glucoronidation     
  K m 5500 5500 [20]  
  n (Hill) 3.2    
  V max in liver 6370    
  V max in tissue 1274    
V UGT2 glucoronidation     
  K m 4000 4000 [20]  
  K i 23000 23000 [20]  
  V max in liver 490    
  V max in tissue 98    
V UGT3 glucoronidation     
  K m 9200 9200 [20]  
  V max in liver 4900    
  V max in tissue 980    
V UGT4 glucoronidation     
  K m 23000 23000 [20]  
  K i 5300 5300 [20]  
  V max in liver 7350    
  V max in tissue 1470    
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Theoretical Biology and Medical Modelling

ISSN: 1742-4682

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