Skip to main content

Table 1 Parameter values and derived quantities for the in vitro experiment

From: A highly pathogenic simian/human immunodeficiency virus effectively produces infectious virions compared with a less pathogenic virus in cell culture

Parameter name Symbol Unit SHIV-KS661 SHIV-#64
Value 95% CI Value 95% CI
Calculated parameters for the continuous approximation of cell and virus harvest
Harvest rate of target and infected cells μ day-1 0.057 0.057
Harvest rate of total and infectious virus c day-1 1.93 1.93
Fitted parameters from separate experiments
Rate of virion infectivity loss c 50 day-1 0.869 0.598–1.141 0.992 0.866–1.117
Degradation rate of virion RNA c RNA day−1 0.091 0.029–0.152 0.160 0.051–0.269
Parameters obtained from simultaneous fit to full in vitro dataset
Rate constant for infections β 50 (TCID50/ml・day)−1 1.49 × 105 7.97 × 10−6–2.46 × 105 1.47 × 104 8.86 × 10−5–2.36 × 104
Death rate of target cells d day−1 6.95 × 10−3 3.89 × 104–5.15 × 102 2.47 × 10−3 1.09 × 103–5.75 × 103
Death rate of infected cells δ day−1 1.48 1.20–1.81 1.56 1.23–1.96
Production rate of total virus p RNA RNA copies・day−1 3.74 × 104 1.94 × 104–6.35 × 104 4.15 × 104 2.67 × 104–6.07 × 104
Production rate of infectious virus p 50 TCID50・day−1 0.322 0.164–0.593 0.028 0.0156–0.0426
Quantities derived from fitted values
Viral burst size (total) p RNA /δ RNA copies 2.52 × 104 1.39 × 104–4.08 × 104 2.65 × 104 2.03 × 104–3.43 × 104
Viral burst size (infectious) p 50/ δ TCID50 0.215 0.127–0.351 0.018 0.0104–0.0280
Malthusian coefficient M 3.08 2.82–3.52 2.59 2.37–2.80
Basic reproductive number (without removal) R 0 19.6 16.5–23.6 13.4 11.4–15.7
Basic reproductive number (with removal) R *0 6.25 5.29–7.51 4.84 4.13–5.62