|
Calculated parameters for the continuous approximation of cell and virus harvest
|
|
Harvest rate of target and infected cells
|
μ
|
day-1
|
0.057
|
―
|
0.057
|
―
|
|
Harvest rate of total and infectious virus
|
c
|
day-1
|
1.93
|
―
|
1.93
|
―
|
|
Fitted parameters from separate experiments
|
|
Rate of virion infectivity loss
|
c
50
|
day-1
|
0.869
|
0.598–1.141
|
0.992
|
0.866–1.117
|
|
Degradation rate of virion RNA
|
c
RNA
|
day−1
|
0.091
|
0.029–0.152
|
0.160
|
0.051–0.269
|
|
Parameters obtained from simultaneous fit to full in vitro dataset
|
|
Rate constant for infections
|
β
50
|
(TCID50/ml・day)−1
|
1.49 × 105
|
7.97 × 10−6–2.46 × 105
|
1.47 × 104
|
8.86 × 10−5–2.36 × 104
|
|
Death rate of target cells
|
d
|
day−1
|
6.95 × 10−3
|
3.89 × 104–5.15 × 102
|
2.47 × 10−3
|
1.09 × 103–5.75 × 103
|
|
Death rate of infected cells
|
δ
|
day−1
|
1.48
|
1.20–1.81
|
1.56
|
1.23–1.96
|
|
Production rate of total virus
|
p
RNA
|
RNA copies・day−1
|
3.74 × 104
|
1.94 × 104–6.35 × 104
|
4.15 × 104
|
2.67 × 104–6.07 × 104
|
|
Production rate of infectious virus
|
p
50
|
TCID50・day−1
|
0.322
|
0.164–0.593
|
0.028
|
0.0156–0.0426
|
|
Quantities derived from fitted values
|
|
Viral burst size (total)
|
p
RNA
/δ
|
RNA copies
|
2.52 × 104
|
1.39 × 104–4.08 × 104
|
2.65 × 104
|
2.03 × 104–3.43 × 104
|
|
Viral burst size (infectious)
|
p
50/
δ
|
TCID50
|
0.215
|
0.127–0.351
|
0.018
|
0.0104–0.0280
|
|
Malthusian coefficient
|
M
|
―
|
3.08
|
2.82–3.52
|
2.59
|
2.37–2.80
|
|
Basic reproductive number (without removal)
|
R
0
|
―
|
19.6
|
16.5–23.6
|
13.4
|
11.4–15.7
|
|
Basic reproductive number (with removal)
|
R
*0
|
―
|
6.25
|
5.29–7.51
|
4.84
|
4.13–5.62
|
