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Table 1 Comparison of mathematically optimized methods for equilibration of human oocytes with 6 mol/L EG

From: Mathematically optimized cryoprotectant equilibration procedures for cryopreservation of human oocytes

Procedure

Concentration parameterization

Goal cell volume

Goal EG Conc. (sf/wf)

Toxicity (J╬▒)

Addition

Piecewise linear

Isotonic

30

396

Addition

Piecewise linear

Not specified

30

32.3

Addition

2-step piecewise constant

Not specified

30

49.5

Addition

3-step piecewise constant

Not specified

30

42.7

Removal

Piecewise linear

Isotonic

0

38.4

Removal

Piecewise linear

Not specified

0

12.1

Removal

2-step piecewise constant

Not specified

0

12.8

Removal

3-step piecewise constant

Not specified

0

12.4