Papillomavirus binding factor (PBF) is an intrinsically disordered protein with potential participation in osteosarcoma genesis, in silico evidence

Table 2 Anatomy and functions of PBF

Domain^a	Disordered or ordered structure^b	Probable phosphorylation sites^c	Probable binding sites to cellular factors^d	Function
I (1–125)	Disordered region (1–72) Ordered region (86–128)	7 sites	1 site (1–22)	Unknown
II (126–215)	Disordered region (131–216)	8 sites	2 sites (166–182; 193–206)	Unknown
III (216–301)	Ordered region (279–302)	11 sites	1 site (277–304)	-Monopartite NLS (PAPRKRKNSVK, 267–277)^e
				-Zinc finger domain (279–302)
				-Rich G-C DNA binding site of HPV type 8[1]
IV (302–483)	Disordered region (304–513)	18 sites	3 sites (320–341; 359–372; 393–416)	-Binding to 14-3-3β (393–398; 444–453). PBF must be phosphorylated at Ser 447, 449 and 451[5].
IV (302–483)	Disordered region (304–513)	18 sites	3 sites (320–341; 359–372; 393–416)	-Sequence recognized by cytotoxic T cells in the context HLA-A24[2].

Parentheses indicate the amino acid residues position. Bold residues indicate the high affinity site. Identified in this paper using: ^aMammoth analysis; ^bIntfold, disEMBL and Metadisorder analyses; ^cNetPhos 2.0; ^dANCHOR; ^ecNLS Mapper, NucPred, and PSORT II analyses.

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