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Table 2 Epitopes predicted in chimeric protein by different parameters based on Bcepred software

From: In silico analysis of chimeric espA, eae and tir fragments of Escherichia coli O157:H7 for oral immunogenic applications

Prediction parameters Epitope positions*
Hydrophilicity 1-14, 25-38, 47-55, 108-115, 128-144, 160-166, 202-219, 222-230, 232-242, 262-268, 283-291, 301-309, 319-329, 392-404, 448-475, 482-490, 512-526, 528-547, 430-446.
Flexibility 4-10, 25-35, 43-51, 104-113, 199-214, 217-226, 279-287, 307-314, 316-325, 389-403, 447-453, 480-485, 539-545.
Accessibility 2-18, 27-42, 45-55, 81-87, 95-101, 113-120, 128-144, 147-155, 157-166,169-177, 179-191, 201-217, 250-259, 276-282, 289-298, 319-331, 340-349, 374-384, 391-401, 430-463, 467-493, 510-551.
Exposed surface 28-42, 251-259, 340-346, 392-398, 450-457, 472-479, 482-490, 520-527, 530-550.
Polarity 32-39, 128-144, 157-164, 249-259, 430-446, 473-480, 510-526, 533-552.
Antigenic propensity 38-44, 112-119, 174-180, 312-319, 352-360, 363-370, 413-419, 498-508.
  1. * Number shows position of amino acids.