Table 1 List of ZFNs cleaving within 3,094 bp located 5' and 3' of the HSV-2 genome context
Zinc Finger Nuclease (ZFN) | Left Fn; triplet- α-Helix | Right Fn; triplet- α-Helix |
|---|---|---|
-target HSV-2-DNA site 5' | Â | Â |
ZFN-unknown-SP-7-1 | Â | Â |
1 aGTCCCCGTCCTGCCGCGCGGGGGCG g 27 | F1; EEANLRR; (GAC) | F1; KRHTLTR;(GCG) |
1 tCAGGGGCAG GACGGCGCGCCCCCGCc 27 | F2; RREHLVR; (GGG) | F2; RREHLVR;(GGG) |
| Â | F3; DPSNLQR; (GAC) | F3; RTDSLPR; (GCG) |
ZFN-unknown-SP-5-1 | Â | Â |
69 gCCCCGCGGCGCGCGGGGGGAGGG g 93 | F1; RGNHLRR; (GGG) | F1; KKDHLHR;(GGG) |
69 cGGGGCGCCG CGCGCCCCCCTCCCc 93 | F2; RTDTLAR; (GCG) | F2; QSAHLKR; (GGA) |
| Â | F3; ERRGLAR; (GCC) | F3; RTEHLAR; (GGG) |
-target HSV-2-DNA site 3' | Â | Â |
ZFN-unknown-SP-6-685 | Â | Â |
154647 gCCCTGCCGCCCGCCCGCCGCCGCC g154672 | F1; RRAHLQN; (GGG) | F1; DGSTLRR;(GCC) |
154647 cGGGACGGCG GGCGGGCGGCGGCGGc154672 | F2; QSTTLKR; (GCA) | F2; DSSVLRR;(GCC) |
| Â | F3; RLDMLAR; (GCG) | F3; ERRGLAR;(GCC) |
ZFN-unknown-SP-6-682 | Â | Â |
154561 gCCCCGCGGCGCGCGGGGGGAGGGG c154586 | F1; RGNHLRR; (GGG) | F1; RNTHLAR;(GGG) |
154561 cGGGGCGCCG CGCGCCCCCCTCCCCg154586 | F2; RTDTLAR; (GCG) | F2; RQDNLG; (GAG) |
| Â | F3; IRHHLKR; (GGT) | F3;QQGNLQL; (TAA) |