Table 2 The 2 ZFNs cleaving >18 bp sequences specifically within the HIV-pol gene
Zinc Finger Nuclease (ZFN) | Left Fn; triplet- α-Helix | Right Fn; triplet- α-Helix |
|---|---|---|
-target HIV-pol-gene 5' | ||
ZFN-unknown-SP-7-1 | Â | Â |
1063 gTTCTGCCTCAGGGATG GAAGGGGTC a 1089 | F1; QGSNLAR;(GAA) | F1; TKSLLAR;(GTC) |
1063 c AAGACGGAG TCCCTACCTTCCCCAGt 1089 1 | F2; QSTTLKR;(GCA) | F2;RREHLVR;(GGG) |
| Â | F3; RGDNLNR;(GAG) | F3;QDGNLGR;(GAA) |
-target HIV-pol-gene 3' | ||
ZFN-unknown-SP-5-1 | Â | Â |
1871 cAACACCACCGCTAG TAAGATTAG t 1895 | F1; AATALRR;(GTT) | F1; RSHNLRL;(TAG) |
1871 g TTGTGGTGG CGATCATTCTAATCa 1895 | F2; EAHHLSR;(GGT) | F2; VRHNLTR;(GAT) |
| Â | F3; IRHHLKR;(GGT) | F3; QQGNLQL;(TAA) |