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Computational modeling of the effects of autophagy on amyloid-β peptide levels

Abstract

Background

Autophagy is an evolutionarily conserved intracellular process that is used for delivering proteins and organelles to the lysosome for degradation. For decades, autophagy has been speculated to regulate amyloid-β peptide (Aβ) accumulation, which is involved in Alzheimer’s disease (AD); however, specific autophagic effects on the Aβ kinetics only have begun to be explored.

Results

We develop a mathematical model for autophagy with respect to Aβ kinetics and perform simulations to understand the quantitative relationship between Aβ levels and autophagy activity. In the case of an abnormal increase in the Aβ generation, the degradation, secretion, and clearance rates of Aβ are significantly changed, leading to increased levels of Aβ. When the autophagic Aβ degradation is defective in addition to the increased Aβ generation, the Aβ-regulation failure is accompanied by elevated concentrations of autophagosome and autolysosome, which may further clog neurons.

Conclusions

The model predicts that modulations of different steps of the autophagy pathway (i.e., Aβ sequestration, autophagosome maturation, and intralysosomal hydrolysis) have significant step-specific and combined effects on the Aβ levels and thus suggests therapeutic and preventive implications of autophagy in AD.

Introduction

Autophagy (from the Greek, autos, which means “self”, and phagein, “to eat”) is an evolutionarily conserved catabolic pathway, which delivers cytoplasmic constituents such as proteins and organelles to the lysosome for degradation and recycling [1,2,3]. Autophagy regulates protein quality, energy balance, and metabolic homeostasis, and furthermore it plays a role in the decision-making of cellular life and death, depending on the context of its activation [2,3,4,5]. The energy molecules and metabolic building blocks such as adenosine triphosphate (ATP) and amino acids, respectively, which are the recycled products of autophagy, regulate the consecutive steps of the autophagy process, i.e., sequestration (or autophagosome formation), autophagosome maturation (autolysosome formation), and intralysosomal hydrolysis, via mammalian target of rapamycin (mTOR) (for amino acids) and AMP-activated protein kinase (AMPK) pathways (for ATP) [6,7,8,9].

Neurons are especially vulnerable to autophagy dysfunction because they rely heavily upon autophagy for preventing the accumulation of toxic substances such as damaged proteins and protein aggregates [10,11,12]. For this, the brain is considered to be the most severely affected organ by the autophagy dysfunction [11, 12]: It is particularly related to the development of neurodegenerative disorders such as Alzheimer’s disease (AD) and Parkinson’s disease (PD) [10, 11, 13,14,15,16,17]. In young (healthy) neurons, autophagy can efficiently deliver the toxic substances along the unusually large architectures of axons and dendrites to lysosomes, which are concentrated in the cell body, while old (deteriorated) neurons have reduced autophagic degradation efficacy. It is becoming increasingly evident that the autophagic degradations of aggregate-prone proteins in neurons are highly substrate-selective [18]. These selective pathways appear to rely on the specific interactions between substrates and autophagy receptors/adaptors to sequester certain substrates within autophagosomes. Then the substrates proceed to the same degradation machinery as non-selective (bulk) autophagy [19,20,21,22]. Furthermore, it has been suggested that modulation of substrate–receptor/adaptor interactions can be considered as a new therapeutic strategy for neurodegenerative disorders [18].

AD, a common form of dementia, is one of the most prevalent neurological disorders associated with aging as its incidence is rapidly growing every year [23, 24]. The neuropathological hallmarks include deposition of extracellular plaques and formation of intracellular neurofibrillary tangles (NFTs). The plaques and NFTs predominantly consist of amyloid-β peptides (Aβ) and tau proteins, respectively. According to the amyloid hypothesis, an accumulation of Aβ is the primary factor for the onset and progression of AD and the rest of the process including the NFT formation is the secondary effects of the Aβ toxicity [25,26,27]. An increased intracellular Aβ level is observed prior to the onset of extracellular plaque formation.

Aβ consists of 36 to 43 amino acids and is intracellularly generated by specific proteolytic cleavage of the amyloid precursor protein (APP), an integral membrane protein which is concentrated in the synapses of neurons. An altered balance between generation, degradation, secretion (from the intra to the extracellular space of a neuron), and clearance (from the extracellular space) of Aβ is responsible for the intracellular accumulation and extracellular plaque formation. It has been reported that the Aβ generation rate is abnormally high in the early and late stages of AD [28]. Aβ is degraded preferentially via autophagy; yet during late stages of AD autophagosomes fail to fuse with lysosomes [28]. In addition, the Aβ secretion rate depends on the autophagy activity [29,30,31]: the secretion rate is reduced in mice lacking autophagy-related gene 7 (Atg7) [30]. On the other hand, the autophagic activity is influenced by the intracellular Aβ concentration [28, 32,33,34]. The Aβ clearance rate in the extracellular space varies with the Aβ concentration in a biphasic manner [35]. The AD patient is associated with a decrease in clearance by roughly 30%, which may lead to toxic levels of Aβ accumulation in the extracellular space over about 10 years [36].

Although many individual mechanisms have been studied for decades, the association of Aβ kinetics with autophagy activity and the roles of autophagy in the pathogenesis of AD remain elusive. In this study, we develop a mathematical model for autophagy with respect to Aβ kinetics, integrating various individual molecular and cellular data sets, in hope of providing a unified framework for understanding the complex dynamics between autophagy and Aβ pathways. Simulations are performed to identify the quantitative relationship between autophagy activity and Aβ kinetics, including the intra and extracellular levels, secretion, clearance, and autophagic degradation. This may provide a starting point for understanding the effects of autophagy on the pathogenesis of AD and implications of pharmacological autophagy modulation for AD therapy and prevention.

Mathematical model

The model assumes a four-compartment description of the autophagy process, including 1) intracellular protein (including normal/abnormal protein and intracellular Aβ), 2) autophagosome, 3) autolysosome, and 4) extracellular Aβ compartments (Fig. 1).

Fig. 1
figure 1

Schematic diagram of the model system. The rounded rectangles with white borders illustrate four compartments: 1) intracellular protein, 2) autophagosome, 3) autolysosome, and 4) extracellular amyloid-β (Aβ) peptide. CS1, CS2, and CS3 denote the concentrations of intracellular resident protein S1, abnormal protein S2, and amyloid-β peptide S3, respectively. Cgi and Cli represent the concentrations of autophagosomes and autolysosomes, respectively, from Si (i = 1, 2, 3). CES3 stands for the extracellular Aβ concentration. Rgi, Rli, Rhi, and Rdi are the specific rates of autophagosome formation, autolysosome formation, intralysosomal hydrolysis, and non-autophagic degradation, respectively, for Si (i = 1, 2, and 3 again). Rsec and Rclr denote respectively the rates of Aβ secretion and clearance. The differential equations describe variations of the concentrations of proteins (Eqs. (1)–(4)), autophagosomes (Eq. (5)), autolysosomes (Eq. (6)), amino acids (Eq. (7)), and ATP (Eq. (8))

Dynamic equations

Intracellular proteins are classified as resident proteins S1 which conduct normal functions in a cell, abnormal proteins S2 including damaged proteins and those abnormally transcribed or translated, and amyloid-β peptide S3. We write the equations for the dynamics of concentrations CS1, CS2, and CS3 of S1, S2, and S3, respectively, in the form:

$$ \frac{d{C}_{\mathrm{S}1}}{dt}=\left(1-\alpha \right){R}_S-\sigma {C}_{\mathrm{S}1}-{R}_{g1}{C}_{\mathrm{S}1}-{R}_{d1}-\beta {C}_{\mathrm{S}1}, $$
(1)
$$ \frac{d{C}_{\mathrm{S}2}}{dt}=\alpha {R}_S+\sigma {C}_{\mathrm{S}1}-{R}_{g2}{C}_{\mathrm{S}2}-{R}_{d2}, $$
(2)
$$ \frac{d{C}_{\mathrm{S}3}}{dt}=\beta {C}_{\mathrm{S}1}-{R}_{g3}{C}_{\mathrm{S}3}-{R}_{d3}-{R}_{sec}{C}_{\mathrm{S}3}, $$
(3)

where RS represents the (total) protein synthesis rate (from DNA) and α is the fraction of S2, namely, S1 and S2 are produced at the rates of (1 – α)RS and αRS, respectively. σ is the rate constant for deterioration of S1 (i.e., transformation from S1 to S2). Rgi and Rdi represent the specific rates of autophagosome formation and the non-autophagic degradation of Si (for i = 1, 2, and 3), respectively. β denotes the rate constant for Aβ generation and Rsec is the Aβ secretion specific rate from the intra to the extracellular space.

The dynamics of the Aβ concentration in the extracellular space CES3 reads:

$$ \frac{d{C}_{\mathrm{ES}3}}{dt}={R}_{sec}{C}_{\mathrm{S}3}-{R}_{clr}{C}_{\mathrm{ES}3}, $$
(4)

where Rclr denotes the specific clearance rate for Aβ in the extracellular space.

Variations of the intracellular autophagosome concentration with time are determined by the difference between the autophagosome formation specific rate Rgi and the autolysosome formation specific rate Rli (i = 1, 2, and 3 for S1, S2, and S3, respectively). With Cgi denoting the concentration of autophagosome originating from Si (i = 1, 2, and 3), the dynamics of the concentration is governed by the following equation:

$$ \frac{d{C}_{\mathrm{gi}}}{dt}={R}_{gi}{C}_{\mathrm{Si}}-{R}_{li}{C}_{\mathrm{gi}}. $$
(5)

The intracellular concentration Cli of autolysosomes originating from Si (i = 1, 2, and 3) is determined by the difference between Rli and the intralysosomal hydrolysis specific rate Rhi (i = 1, 2, and 3). The equation governing the dynamics takes the form:

$$ \frac{d{C}_{\mathrm{li}}}{dt}={R}_{li}\left(t-\tau \right)\;{C}_{\mathrm{gi}}\left(t-\tau \right)-{R}_{hi}{C}_{\mathrm{li}}. $$
(6)

Note that the autolysosome concentration at time t is affected by the autophagosome concentration at time tτ, earlier by the delay time τ, which is taken to be 8 min (τ = 480 s) [37,38,39].

The dynamics of intracellular amino acids, the concentration of which is denoted by Ca reads:

$$ \frac{d{C}_{\mathrm{a}}}{dt}={\mu}_a{R}_{hi}\sum \limits_{i=1}^3{C}_{\mathrm{li}}+{\mu}_d\sum \limits_{i=1}^3{R}_{di}+{R}_a-{\mu}_s{R}_S. $$
(7)

The first and second terms on the right-hand side correspond to the supply of amino acids due to the autophagic intralysosomal hydrolysis and non-autophagic protein degradation, respectively, with appropriate constants μa and μd describing the average numbers of amino acids produced from autophagic and non-autophagic degradation, respectively. The third term represents the rate of amino acid supply from extracellular fluid into cells that is assumed to be proportional to the metabolic demand (i.e., protein synthesis rate RS) and the loss of protein (i.e., secretion rate of Aβ, given by RsecCS3) such that Ra = μcRS + μβRsecCS3 with appropriate constants μc and μβ. The last term describes the reduction of amino acids due to protein synthesis with the constant μs, the average number of amino acids in a protein molecule.

The dynamic equation for intracellular ATP concentration CA reads:

$$ \frac{d{C}_{\mathrm{A}}}{dt}={v}_a{R}_{hi}\sum \limits_{i=1}^3{C}_{\mathrm{li}}+{v}_d\sum \limits_{i=1}^3{R}_{di}+{R}_A-{v}_s{R}_S $$
(8)

where νa and νd are the average numbers of ATP molecules produced from autophagic degradation and from non-autophagic degradation, respectively. The net intracellular ATP generation rate RA is assumed to be RA = νcRS + νβRsecCS3 that is associated with the metabolic demand and the loss of protein, with appropriate constants νc and νβ. The last term corresponds to the reduction of ATP due to protein synthesis, where νs gives the average number of ATP molecules in a protein.

n average protein molecule in a cell is assumed to be composed of 500 amino acid residues; in other words, 500 amino acids are consumed in unit protein synthesis (i.e., μs = 500). Considering that elongation of one amino acid during translation requires approximately four ATP molecules, we have assumed that 2000 ATP molecules are required for the synthesis of a protein (νs = 2000). However, the numbers of amino acids and ATP molecules per degradation of one protein via autophagic or non-autophagic protein degradation have been set to be less than those required in the protein synthesis, because the efficacy of protein recycling is expected to be less than 100%; this yields μa = μd = μβ = νa = νd = νβ = 300, μc = 200, and νc = 1700.

Details of the autophagy-related rates in Eqs. (1) to (8) are given in the following subsections. The parameters are summarized in Table 1.

Table 1 Parameters in computer simulations

Autophagosome formation

We take the autophagosome formation specific rates Rgi from Si (for i = 1, 2, and 3), which depend on the intracellular concentrations CS3 of Aβ [28, 32,33,34], CA of ATP [40, 41], and Ca of amino acids [42] as follows:

$$ {R}_{g1}\left({C}_{\mathrm{S}3},{C}_{\mathrm{a}},{C}_{\mathrm{A}}\right)={r}_{g1}\left({\omega}_g{C_{\mathrm{S}3}}^{\zeta_g}+{\psi}_g{C}_{\mathrm{S}3}+1\right)\frac{{C_{\mathrm{A}}}^4}{{C_{\mathrm{A}}}^4+{k_g}^4}\frac{{p_g}^{12}}{{C_{\mathrm{A}}}^{12}+{p_g}^{12}}\frac{{a_g}^8}{{C_{\mathrm{a}}}^8+{a_g}^8}\left(1+{\gamma}_g{e}^{-{\xi}_g{C}_{\mathrm{a}}}\right), $$
(9)
$$ {R}_{g2}\left({C}_{\mathrm{S}3},{C}_{\mathrm{a}},{C}_{\mathrm{A}}\right)={r}_{g2}\left({\omega}_g{C_{\mathrm{S}3}}^{\zeta_g}+{\psi}_g{C}_{\mathrm{S}3}+1\right)\frac{{C_{\mathrm{A}}}^4}{{C_{\mathrm{A}}}^4+{k_g}^4}\frac{{p_g}^{12}}{{C_{\mathrm{A}}}^{12}+{p_g}^{12}}\left(1+{\gamma}_g{e}^{-{\xi}_g{C}_{\mathrm{a}}}\right), $$
(10)
$$ {R}_{\mathrm{g}3}\left({C}_{\mathrm{S}3},{C}_{\mathrm{a}},{C}_{\mathrm{A}}\right)={r}_{g3}\left({\omega}_g{C_{\mathrm{S}3}}^{\zeta_g}+{\psi}_g{C}_{\mathrm{S}3}+1\right)\frac{{C_{\mathrm{A}}}^4}{{C_{\mathrm{A}}}^4+{k_g}^4}\frac{{p_g}^{12}}{{C_{\mathrm{A}}}^{12}+{p_g}^{12}}\left(1+{\gamma}_g{e}^{-{\xi}_g{C}_{\mathrm{a}}}\right), $$
(11)

where rgi is the rate constant for autophagosome formation from Si (for i = 1, 2, and 3), with appropriate constants ωg, ζg, ψg (for Aβ), kg, pg (ATP), ag, γg, and ξg (amino acids).

Intracellular Aβ affects the mTOR signaling, which negatively regulates autophagy induction, exhibiting a nonlinear relationship: The mTOR activity increases (i.e., suppressing autophagosome formation) with the Aβ level until reaching a certain threshold (~ 0.5 μM) and then the activity gradually decreases (restoring autophagosome formation) above the threshold concentration [28, 32,33,34]. This nonlinear relationship has been included in Eqs. (9)–(11) as a simple algebraic equation in the form of \( {\omega}_g{C_{\mathrm{S}3}}^{\zeta_g}+{\psi}_g{C}_{\mathrm{S}3}+1 \).

The remaining part of the right-hand side contains the ATP and amino acid dependency of the autophagosome formation step. Under normal conditions, it appears that S2 and S3, abnormal proteins and Aβ, are preferentially degraded by autophagy. However, as the intracellular energy/nutrient reduces due to, e.g., starvation or increased metabolic demand, all the proteins (S1, S2 and S3) are degraded non-selectively for the rapid supply of essential energy molecules (e.g., ATP) and metabolic building blocks (i.e., amino acids) [21, 22, 43, 44]. Therefore, it is assumed in this model that the autophagosome formation rate from resident proteins S1, which is lower than that from abnormal proteins and Aβ (S2 and S3) under normal conditions, becomes gradually equal to those of S2 and S3 as the amino acid concentration is decreased [45,46,47,48].

Autolysosome formation and intralysosomal hydrolysis

The autolysosome formation specific rate Rli reads (i = 1, 2, and 3 for S1, S2, and S3)

$$ {R}_{li}\left({C}_{\mathrm{A}}\right)={r}_{li}\frac{{C_{\mathrm{A}}}^4}{{C_{\mathrm{A}}}^4+{k_l}^4}\frac{{p_l}^{12}}{{C_{\mathrm{A}}}^{12}+{p_l}^{12}}, $$
(12)

where rli denotes the rate constant for autolysosome formation from Si with appropriate constants kl and pl for ATP, based on biological experiments [40, 41].

The intralysosomal hydrolysis specific rate Rhi is taken as a function of the intracellular ATP concentration (i = 1, 2, and 3):

$$ {R}_{hi}\left({C}_{\mathrm{A}}\right)={r}_{hi}\frac{{C_{\mathrm{A}}}^{\delta_h}}{{C_{\mathrm{A}}}^{\delta_h}+{k_h}^{\delta_h}}, $$
(13)

with appropriate exponent δh and constant kh for ATP, where rhi is the rate constant for intralysosomal hydrolysis [40, 41]. Further details of the equations for autolysosome formation and intralysosomal hydrolysis can be found in literature [4, 9, 49, 50].

Secretion and clearance of amyloid-β

Considering that Aβ secretion from the intra to extra cellular space of a neuron is positively correlated with the autophagy induction level [29,30,31], we assume the Aβ secretion specific rate Rsec to be proportional to the degree of amino acid- and ATP-dependent autophagosome induction, as defined in Eqs. (9)–(11), with an appropriate constant rsec:

$$ {R}_{sec}\left({C}_{\mathrm{a}},{C}_{\mathrm{A}}\right)={r}_{sec}\frac{{C_{\mathrm{A}}}^4}{{C_{\mathrm{A}}}^4+{k_g}^4}\frac{{p_g}^{12}}{{C_{\mathrm{A}}}^{12}+{p_g}^{12}}\left(1+{\gamma}_g{e}^{-{\xi}_g{C}_{\mathrm{a}}}\right). $$
(14)

The concentration-dependent biphasic Aβ clearance rate Rclr in the extracellular space is assumed, on the basis of biological experiments [35, 36, 51], to take the form:

$$ {R}_{clr}\left({C}_{\mathrm{ES}3}\right)={r}_{clr}\left({C}_{\mathrm{ES}3}+{\omega}_{ext}\right), $$
(15)

where rclr denotes the rate constant for Aβ clearance, with an appropriate constant ωext. The rate of Aβ clearance varies with the concentration according to the measurement on Alzheimer’s mouse model [35]: While the half-life is very short at high concentrations of extracellular Aβ, it grows longer as the concentration decreases. Equation (15) captures qualitatively this biphasic nature of Aβ clearance [35] and its value lies within a reasonable range consistent with the state-of-the-art measurements [36, 51].

Protein synthesis and non-autophagic degradation

The (total) protein synthesis rate RS which depends on intracellular concentrations Ca of amino acids and CA of ATP reads [52].

$$ {R}_S\left({C}_{\mathrm{a}},{C}_{\mathrm{A}}\right)=\Big\{{\displaystyle \begin{array}{c}{r}_s\frac{C_{\mathrm{a}}}{C_{\mathrm{a}}+{k}_s}\frac{\exp \left[{C}_{\mathrm{A}}\right]-1}{\exp \left[{C}_{\mathrm{A}}^{(m)}\right]-1}\kern1em \mathrm{for}\ {C}_{\mathrm{A}}<{C}_{\mathrm{A}}^{(m)}\\ {}{r}_s\frac{C_{\mathrm{a}}}{C_{\mathrm{a}}+{k}_s}\kern6em \mathrm{for}\ {C}_{\mathrm{A}}\ge {C}_{\mathrm{A}}^{(m)}\end{array}} $$
(16)

with appropriate constant ks for amino acid, where \( {C}_{\mathrm{A}}^{(m)} \) is the ATP concentration corresponding to the maximal protein synthesis rate and rs denotes the rate constant for the protein synthesis. Further details of the protein synthesis can be found in literature [4, 9, 49, 50].

The non-autophagic protein degradation machinery such as the ubiquitin-proteasome system has been considered in the model. We assume that the amount of protein degradation by autophagy constitutes 80% of the total amount of protein degradation and the non-autophagic protein degradation machinery is responsible for the remaining 20% [53]. Accordingly, we take the rate of non-autophagic degradation Rdi (i = 1, 2, and 3) to be 25% of autophagic degradation:

$$ {R}_{di}=\frac{1}{4}{R}_{hi}{C}_{\mathrm{li}}. $$
(17)

Results

Aβ kinetics under normal and pathological conditions

In Fig. 2, the relation of intracellular (CS3) and extracellular (CES3) Aβ levels with the respective Aβ fluxes under normal conditions (i.e., for basal parameter values) are shown, providing kinetic and dynamic insights into the Aβ regulation. As illustrated in Fig. 1, CS3 (the second row of the first column) is determined by the difference between influx (i.e., Aβ generation flux, denoted by Fgen, the concentration of Aβ generated per unit time given in units of mM/s) and efflux rates such as autophagic sequestration Fseq (the concentration of intracellular Aβ sequestered into autophagosomes per unit time, i.e., Fseq = Rg3CS3), non-autophagic degradation Fnap (the concentration of intracellular Aβ degraded via the non-autophagic mechanism per unit time, i.e., Fnap = Rd3), and secretion Fsec (the concentration of intracellular Aβ secreted from the inside to outside of a neuron per unit time, i.e., Fsec = RsecCS3). CES3 (the third row of the second column) is governed by Fsec and the clearance flux Fclr (the concentration of Aβ removed from the extracellular space per unit time, i.e., Fclr = RclrCES3).

Fig. 2
figure 2

The basal steady-state Aβ concentrations and fluxes. CS3 and CES3 represent the concentrations of intracellular (IC) and extracellular (EC) Aβ, respectively. Fgen, Fseq, and Fnap denote the Aβ generation, sequestration (the first step of autophagic degradation, i.e., autophagosome formation), and non-autophagic degradation fluxes, respectively; Fsec and Fclr the secretion (from the IC to the EC space) and Aβ clearance (in the EC space) fluxes, respectively

Figures 3 and 4 compare values of CS3 and CES3, respectively, under the normal, early stage (i.e., abnormal increase in Aβ generation), and late stage AD (i.e., increased Aβ generation together with decreased autophagic lysosomal degradation) conditions [28]. The simulations have been performed with the basal value β(0) of the Aβ generation rate constant, i.e., β = β(0), for the normal condition, while data for the early and late stage AD conditions have been obtained at an extremely high Aβ generation rate, β = 100 × β(0). Further, in the late stage case, the specific rate constants of autolysosome formation and intralysosomal hydrolysis have been set to be 10% of the basal values, i.e., rl3 = 0.1 × rl3(0) and rh3 = 0.1 × rh3(0).

Fig. 3
figure 3

Intracellular Aβ concentrations under normal and pathological conditions. The intracellular Aβ concentration CS3 displays oscillatory behaviors depending on the parameters. The basal value of Aβ generation rate constant (i.e., β = β(0)) has been used for the normal condition while β = 100 × β(0) has been used for the early stage AD. For the late stage AD, the specific rate constants of autolysosome formation and intralysosomal hydrolysis have been set equal to rl3 = 0.1 × rl3(0) and rh3 = 0.1 × rh3(0), retaining the high Aβ generation rate as the early state AD. The results in the second column were obtained under 20-fold increase in the autophagosome formation rate constant (rg3 = 20 × rg3(0)) with others the same as those in the first column

Fig. 4
figure 4

Extracellular Aβ concentrations under normal and pathological conditions. The extracellular Aβ concentration CES3 displays oscillatory behaviors depending on the parameters. The basal value of the Aβ generation rate constant (β = β(0)) was used for the normal condition while a high Aβ generation rate β = 100 × β(0) has been used for the early and late stage AD. For the late stage AD, the specific rate constants of autolysosome formation and intralysosomal hydrolysis have been set equal to rl3 = 0.1 × rl3(0) and rh3 = 0.1 × rh3(0), in addition to the high Aβ generation rate. The results in the second column were obtained under 20-fold increase in the autophagosome formation rate constant (rg3 = 20 × rg3(0)), with other parameters remaining unchanged

It is observed that CS3 and CES3 are significantly higher in AD conditions than in the basal condition—CS3 is higher at the early stage than at the late stage AD (Fig. 3) while CES3 is higher at the late stage AD (Fig. 4). In both pathological conditions, autophagy induction (i.e., a 20-fold increase in the autophagosome formation rate constant: rg3 = 20 × rg3(0)) significantly reduces CS3 and CES3. In addition, the early and late stage AD exhibit asymmetric oscillating patterns. CS3 increases gradually and then drops rapidly; conversely, CES3 increases rapidly and drops gradually. Under the basal condition they exhibit relatively symmetrical oscillation patterns.

Both Aβ secretion flux Fsec and clearance flux Fclr are significantly promoted in the early and late stage AD cases compared to those in the basal condition (the first column of Fig. 5). The peaks of Fsec in early AD are sharper and higher but stay at the near-zero rate for a longer period than in late AD. In contrast, Fclr exhibits higher peaks in late AD than in early AD. Autophagy induction (i.e., rg3 = 20 × rg3(0)) significantly reduces those fluxes, close to the basal levels.

Fig. 5
figure 5

Aβ secretion and clearance fluxes in normal and pathological conditions. Fsec and Fclr denote the Aβ secretion flux (from the intracellular to the extracellular space) and Aβ clearance flux in the extracellular space, respectively. The results in the second column have been obtained under 20-fold increase in the autophagosome formation rate constant (rg3 = 20 × rg3(0)), with other parameters kept unchanged

In what follows, autophagy dynamics corresponding to the normal and AD conditions are presented, including steady-state concentrations of autophagosome, autolysosome, and autophagic fluxes.

Dynamics of autophagy and implications in the Aβ regulations

Protein sequestration (i.e., autophagosome formation) flux Fseq, autophagosome maturation (i.e., autolysosome formation) flux Fmat, and intralysosomal hydrolysis flux Fhyd in both early and late stage AD are significantly increased compared with those on the basal condition (the first, third, and fifth rows of Fig. 6). The peaks of Fseq and Fmat in early stage AD are sharper and higher than those in the late stage. The steady-state concentrations of autophagosomes and autolysosomes, Cg3 and Cl3, in the AD cases are greater than those in the basal condition case: the values at the late stage of AD are about ten times greater than those at the early stage (the second and fourth rows of Fig. 6).

Fig. 6
figure 6

Dynamics of autophagy. Fseq, Fmat, and Fhyd denote fluxes of protein sequestration (i.e., autophagosome formation), autophagosome maturation (i.e., autolysosome formation), and intralysosomal hydrolysis steps, respectively. Cg3 and Cl3 are the autophasosome and autolysosome concentrations in Aβ, respectively. Yellow, cyan, and purple lines plot results of autophagy induction (i.e., rg3 = 20 × rg3(0)) in the cases of the basal condition, early stage AD, and late stage AD, respectively. Green, blue, and red lines plot results from simulations with rg3(0) in the same three cases (basal, early stage AD, and late stage AD), respectively

In the cases of early and late stage AD, autophagy induction (i.e., rg3 = 20 × rg3(0)) significantly decreases Fseq and Fmat, while it increases Fhyd (the first, third, and fifth rows of the second and third columns of Fig. 6). The steady-state autophagosome concentration Cg3 is decreased while the autolysosome concentration Cl3 is increased upon autophagy induction (the second and fourth rows of the second and third columns of Fig. 6). Under the basal condition, the oscillatory patterns of autophagic fluxes and steady-state concentrations of autophagosomes and autolysosomes are not significantly affected by the autophagy induction, compared to the AD cases.

As shown above, autophagy induction (i.e., \( {r}_{g3}=20\times {r}_{g3}^{(0)} \)) significantly reduces CS3 and CES3. Increasing rg3 beyond \( 20\times {r}_{g3}^{(0)} \) reduces the Aβ levels further, until they reach basal levels. However, the required value of rg3 to bring the basal levels may vary depending on the stage of AD and the activities of the other autophagic steps such as autophagosome maturation (i.e., autolysosome formation) and intralysosomal hydrolysis.

Figure 7 presents a three-dimensional surface plot, exhibiting step-specific and combined effects of the autophagy pathway on Aβ levels for a moderately high Aβ formation rate β/β(0) = 10 (the first column) and an extremely high formation rate β/β(0) = 100 (the second column). The vertical axis measures the autophagosome formation rate relative to its normal value (i.e., rg3/rg3(0)) and the two horizontally placed axes represent the autolysosome formation and the intralysosomal hydrolysis rates relative to the normal values, spanning the range from highly induced activity (rl3/rl3(0) = rh3/rh3(0) = 30) to normal (rl3/rl3(0) = rh3/rh3(0) = 1) and extremely reduced activity (rl3/rl3(0) = rh3/rh3(0) = 0.1). The surfaces designate time-averaged intracellular Aβ concentration 〈CS3〉 (top) and extracellular Aβ concentration 〈CES3〉 (bottom) for basal parameter values (i.e., under normal conditions); regions above and below the surface correspond to Aβ concentrations lower and higher than the basal values, respectively.

Fig. 7
figure 7

Aβ concentrations depending upon activities of three autophagy steps. The surfaces specify time-averaged intracellular Aβ concentration 〈CS3〉 (first row) and extracellular Aβ concentration 〈CES3〉 (second row) for basal parameter values; regions above and below the surfaces correspond to Aβ concentrations lower and higher than the basal values. The first and the second columns correspond to β/β(0) = 10 and β/β(0) = 100, respectively. Computations were performed with rl3/rl3(0) and rh3/rh3(0) varied in increments and the mixed cubic and quintic spline interpolation applied. On the surfaces in purple the Aβ concentrations display oscillations while oscillations are absent on the green surfaces

For both Aβ synthesis rates (β/β(0) = 10 and 100), 〈CS3〉 and 〈CES3〉 decrease with rg3 in a log-normal manner, \( {\left\langle C\right\rangle}_{r_{g3}/{r}_{g3}^{(0)}=x}=\left(\frac{\gamma }{x\sigma \sqrt{2\pi }}\right)\exp \left[-{\left(\log\ x-\mu \right)}^2/2{\sigma}^2\right] \), where 〈C〉 denotes 〈CS3〉 or 〈CES3〉 and γ, σ, and μ are adjustable parameters (Fig. 8). When rl3 is decreased from 1 to 0.1, 〈CS3〉 decreases while 〈CES3〉 increases. In contrast, when rl3 > 1, the concentrations are relatively independent of rl3. The effects of rh3 generally follow the trend.

Fig. 8
figure 8

Log-normal relations between average Aβ concentrations and rg3/rg3(0). Log-log plots of 〈CS3〉 (top) and 〈CES3〉 (bottom) versus rg3/rg3(0) for rl3/rl3(0) = rh3/rh3(0) = 1 (left column) and 0.1 (right column). Data were obtained at β/β(0) = 10. Squares indicate average values obtained via simulations and lines depict the least square fit of the log-normal relation

The surface shape of Fig. 7 reflects the combined effects of the three-autophagy steps. A greater vale of rg3 is required to return to basal values in the case β/β(0) = 100 compared with the case β/β(0) = 10. At rl3/rl3(0) < 1 and rh3/rh3(0) < 1 both concentrations change greatly compared with the case rl3/rl3(0) > 1 and rh3/rh3(0) > 1, indicating that reduction of autolysosome formation and/or intralysosomal hydrolysis has greater impact on the Aβ concentrations than promotion of these steps. Above rh3/rh3(0) = ~ 45.2 (for β/β(0) = 10) and rh3/rh3(0) = ~ 11.1 (for β/β(0) = 100), the oscillations of proteins (CS1, CS2, CS3, and CES3), ATP (CA), and amino acids (Ca) disappear, converging to stationary values (green surfaces in Figs. 7 and 9). In the stationary region, the effects of rl3/rl3(0) and rh3/rh3(0) are minimal, as manifested by the flatness of the green surface.

Fig. 9
figure 9

Effects of rl3 and rh3 on Aβ concentrations. Average intracellular Aβ concentration 〈CS3〉 (first and third rows) and extracellular Aβ concentration 〈CES3〉 (second and fourth rows) at β/β(0) = 10 (upper two rows) and β/β(0) = 100 (lower two rows), depending upon changes of rl3/rl3(0) (first column), rh3/rh3(0) (second column), and rl3/rl3(0) and rh3/rh3(0) together (third column). At data points in purple, oscillations of Aβ concentrations are observed; at green data points, concentrations are stationary

Discussion

In this study we have investigated via modeling and simulations how autophagy activity affects Aβ kinetics such as the intra and extracellular levels, secretion, clearance, and autophagic degradation. The mathematical model has been extended from the multi-compartment autophagy model originally developed by Han and Choi [4, 9, 49, 50] to the one with Aβ kinetics incorporated by accommodating the current working hypothesis [29,30,31] and the experimental mechanistic studies [28,29,30,31,32,33,34,35,36, 51] on the relationship between autophagy activity and Aβ kinetics. Such multi-compartment frameworks [4, 9, 49, 50] are especially useful for testing biological hypotheses regarding the selective autophagy including Aggrephagy (i.e., autophagic degradation of protein aggregates), Mitophagy (for mitochondria), and Xenophagy (for microbes) [54] because the model can be easily modified easily to incorporate new substrates for selective degradation in each compartment (see Fig. 1). This approach can be further improved by including detailed mathematical descriptions of autophagy-related cellular signaling pathways, which have been extensively explored in recent years [55,56,57,58,59].

The analysis began with the profiles of Aβ fluxes governing the intracellular and extracellular Aβ concentrations under the normal conditions. As shown in Fig. 2, the intracellular Aβ concentration is determined by the difference between influx (i.e., Aβ generation flux) and efflux rates of autophagic sequestration, non-autophagic degradation, and Aβ secretion, while the extracellular Aβ concentration is governed by Aβ secretion and clearance. This provides an overview of the system—how the Aβ levels might be determined, giving the idea of how to maintain normal Aβ levels against pathological conditions. Promoting autophagic sequestration flux (i.e., autophagy induction) would significantly reduce the intracellular and extracellular Aβ concentrations for the early and the late stage AD (Figs. 3 and 4). Interestingly, the intracellular concentration is higher in early stage than late stage AD, while extracellular concentration is higher in late stage AD. Aβ secretion and clearance fluxes are promoted in the early and late stage AD compared to the normal condition (Fig. 5). In both pathological conditions, promoting autophagic sequestration efficiently decreases the Aβ secretion and clearance fluxes.

In the examination of autophagy dynamics under normal and pathological conditions (Fig. 6), the autophagic fluxes and the concentrations of autophagosome (Cg3) and autolysosome (Cl3) in both early and late stage AD are significantly increased than in the basal condition. Cg3 and Cl3 are about ten times greater in late stage AD than in early stage AD. This implies that at the late stage AD the increased concentrations due to reduced maturation and intralysosomal hydrolysis may clog neurons, which would further reduce the autophagic Aβ degradation efficacy. Under normal conditions the basal autophagy level is sufficient for removing intracellular Aβ as the mTOR activity is tightly regulated. However, during early and late stage of AD, an increase in soluble Aβ levels leads to mTOR hyperactivity, which should in turn suppress autophagosome formation (i.e., reduced Aβ sequestration) (for details see Autophagosome formation in Mathematical model). Reduced autophagosome formation would increase further the Aβ levels, creating a vicious cycle.

The influence of each autophagic step on the intracellular and the extracellular Aβ concentrations (CS3 and CES3) was examined, providing insight into disease and potential effects of drugs targeting specific steps in the autophagic pathway. The autophagosome formation activity plays a significant role in regulating average values of CS3 and CES3 via a log-normal relation: promoting the autophagosome formation step decreases both Aβ levels. As the autolysosome formation and intralysosomal hydrolysis rates are decreased, as expected in late stage AD, CS3 decreases but CES3 increases. It is thus disclosed that the progress from early to late stage AD leads to higher CES3 levels, which could contribute to the deposition of extracellular plaques. On the other hand, CS3 decreases along the pathway to late stage AD (i.e., autophagic Aβ degradation is defective in addition to the increased Aβ generation).

The model has reproduced successfully the oscillatory behavior of autophagy activity concerning the autophagy-related fluxes and the concentrations of Aβ, autophagosomes, and autolysosomes (Figs. 2-6). Such simulated “autophagy oscillations” are qualitatively similar to those observed in biological experiments [60,61,62,63,64,65,66,67,68,69]. However, mechanisms underlying the phenomena have only begun to be explored [68,69,70]. For instance, the oscillations might be tightly controlled via the autophagy-related signaling pathways to keep the autophagy activity within physiological levels that is important for cellular homeostasis. The simulation results presented here exhibit two interesting features: 1) In the early- and late-stage AD, oscillations of CS3 and CES3 exhibit asymmetric patterns while they are symmetric under the basal condition. 2) Above certain activity levels of autolysosome formation (measured by rl3) and intralysosomal hydrolysis (rh3) for Aβ, there disappear oscillations of proteins (CS1, CS2, CS3, and CES3), ATP (CA), and amino acids (Ca).

These findings are expected to be useful for the design of future studies and may give insight to maintaining physiological regulation of the Aβ levels. Defects arising in different steps of the autophagy process would influence in a different way the Aβ kinetics, which will give rise to distinct AD pathology. This suggests that pharmacological modulations of the different autophagy steps may have different implications for AD therapy and prevention.

Conclusions

A mathematical model of autophagy and Aβ metabolism has been developed by integrating experimental knowledge of individual mechanisms. It has been observed that the different steps of the autophagy pathway have different effects on the Aβ levels. Promotion of Aβ sequestration has led to a reduction of both intracellular and extracellular Aβ, while suppression of autophagosome maturation and intralysosomal hydrolysis has had opposing effects, increasing intracellular and decreasing extracellular Aβ. The model thus predicts that modulations of different steps have significant step-specific and combined effects on Aβ levels, suggesting therapeutic and preventive implications of autophagy on AD.

Methods

A mathematical model is developed to examine roles of autophagy in modulating Aβ kinetics. The model includes a nonlinear relationship between autophagy activity and intracellular and extracellular Aβ levels. Autophagy degrades intracellular Aβ and influences the Aβ secretion from the inside to the outside of the neuron (i.e., extracellular space) and the concentration-dependent biphasic Aβ clearance in the extracellular space. Conversely, the intracellular Aβ level regulates the autophagy induction step (i.e., autophagosome formation or protein sequestration). The dynamics of these relations are described by twelve coupled differential equations which are solved via the 5th order Runge-Kutta method for very high precision. Mixed spline interpolation has been used to produce the three-dimensional surface plots of the Aβ concentrations.

Availability of data and materials

Not applicable.

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Acknowledgements

The authors thank James Anderson for helpful discussion. The Linux cluster at the Theory Group for Emergence and Complexity (http://tec.snu.ac.kr) was employed.

Funding

K.H. acknowledges support by the Intramural Research Program of the NIH, National Heart, Lung and Blood Institute. K.H. was supported in part by a grant from the KRIBB Research Initiative Program (Korean Biomedical Scientist Fellowship Program), Korea Research Institute of Bioscience and Biotechnology, Republic of Korea. MYC acknowledges support from the National Research Foundation of Korea through the Basic Science Research Program (Grant No. 2019R1F1A1046285).

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KH and MYC designed the study. KH and SHK performed the computations. All participated in the analysis and interpretation of the results and wrote the manuscript.

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Correspondence to Kyungreem Han or MooYoung Choi.

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Han, K., Kim, S. & Choi, M. Computational modeling of the effects of autophagy on amyloid-β peptide levels. Theor Biol Med Model 17, 2 (2020). https://doi.org/10.1186/s12976-020-00119-6

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